摘要
以SDS正丁醇-正庚烷-水组成的微乳体系作为展开剂,通过聚酰胺薄层层析,探讨不同类型微乳液对葛根异黄酮分离检测效果的影响。结果表明,选择含水量为70％的微乳液作为展开剂,分离效果明显,一次检测出12个黄酮化合物;与以三氯甲烷-甲醇-水(7:2．5:0．25)为展开剂的硅胶薄层相比,微乳薄层色谱对葛根异黄酮的检测效果和灵敏度显著提高,为实验研究中常规检测提供了简便快捷的方法。
The study adopted polyamide thin layer chromatography with different sodium dodecyle suphate/n-C4H9OH/n-C7H16/H2O micro-emulsions as its mobile phase to isolate and detect isoflavonoids in Pueraria. The results indicated that polyamide thin layer chromatography with the micro-emulsion whose water content was 70% used as the mobile phase performed best in isoflavonoids isolation and detection of pueraria and it could detect twelve isoflavonoids one time ;compared with silica gel thin layer chromatography with chloroform-methanol-water （7 ： 2.5 ： 0.25） as its mobile,polyamide thin layer chromatography with microemulsion as its mobile phase presented a significantly increased sensitivity and thus it could be used as one convenient and fast way in the routine detection in experiment and research.
出处
《西北植物学报》
CAS
CSCD
北大核心
2006年第4期819-822,共4页
Acta Botanica Boreali-Occidentalia Sinica
基金
西北农林科技大学2005年度青年学术骨干支持计划项目教育部新世纪优秀人才支持计划项目
关键词
葛根异黄酮
分离鉴定
薄层色谱
微乳液
isoflavonoids
Pueraria
isolation
detection
thin layer chromatography
microemulsion
