摘要
目的:研究白果清蛋白(Ginkgoalbumin,GA)对S180肿瘤的抑制活性,并探讨其抗肿瘤作用机制。方法:采用质谱法测定了白果清蛋白GAⅡa的分子量;采用D-脱氧核糖法和硝基蓝四唑(NBT)光化还原法研究了GA抗氧化活性;观察GA的体内抗S180肿瘤作用,用甲基噻唑四唑MTT法测定GA体外对S180细胞的抑制生长作用。结果:GA的分子量为29248u;能抑制体内S180实体瘤和体外S180肿瘤细胞的生长,具有较强的体外清除OH及O2的能力。结论:抑制S180肿瘤生长的作用可能与其抗氧化活性有关。
Objective： To study the anti-tumor activity of ginkgo albumin （GA） and its mechanism. Method： Molecular weight of GA Ⅱ a was detected by MS （mass spectrum）. The antioxidative effect of GA was studied by deoxyribonucleic acid method and nitroblue tetrazolium （NBT） photo-reduction, and the anti-tumor activity of GA on S180 sarcoma was studied both in vivo and in vitro. Results： Molecular weight of GAⅡ a was 29 248u. GA had strong scavenging effect on superoxide radical and hydroxyl radical. The growth of S180 both in vitro and in vivo was inhibited significantly by GA. Conclusion： The anti-tumor activity of GA was probably related with its antioxidative effect.
出处
《营养学报》
CAS
CSCD
北大核心
2006年第3期259-262,共4页
Acta Nutrimenta Sinica
基金
湖北省"十五"攻关资助项目(No.2001AA204B02)
关键词
白果清蛋白
抗氧化
抗肿瘤
ginkgo albumin
antioxidation
anti-tumor
