摘要
在ＭＳ＋ＢＡ１０ｍｇ／Ｌ＋ＮＡＡ５０ｍｇ／Ｌ＋２，４＿Ｄ０＼＾５ｍｇ／Ｌ＋蔗糖２０ｇ／Ｌ的固体培养基上，苹果品种嗄拉试管苗叶片经７天暗培养产生胚性细胞。将具有胚性细胞的叶片转入ＭＳ＋ＢＡ１０ｍｇ／Ｌ＋蔗糖２０ｇ／Ｌ的培养基上暗培养４０天，６５％的叶片直接发生体细胞胚，体细胞胚在同种培养基上萌发，长成小植株。
Embryogenic Cells Were induced from in vitro leaves of apple cultivar `Gala' after 7 days in the dark on MS medium supplemented with 1 0 g/L BA, 5 0mg/L NAA, 0 5mg/L 2,4_D and sucrose 20 0g/L. Sixty five percent of the leaves regenerated somatic embryos directly when transferred to MS medium with 1 0mg/L BA and sucrose 20 0g/L in the dark for 40 days. The regenerated embryos were going to germinate and develop into shoots on the same medium.
出处
《园艺学报》
CAS
CSCD
北大核心
1996年第3期241-245,共5页
Acta Horticulturae Sinica
