摘要
利用ISSR分子标记技术对17份菠萝种质进行了基因组DNA多态性分析。从62个ISSR引物中筛选出6个多态性引物用于PCR扩增,共扩增出70条DNA条带,其中多态性条带44条,所占比例为62.86%,平均每个引物扩增的DNA条带的数目为11.67条。6个品种具有特异性的扩增带,可作为种质鉴定的依据。根据ISSR扩增结果建立的UPGMA聚类图,在距离系数约0.1处可把供试的17份菠萝种质分为3大类。
The polymorphism of 17 Pine apple cultivars were analyzed by inter-simple sequence repeat（ISSR）.6 primers were selected from 62 ISSR primers, and a total of 70 DNA bands were amplified, 44 of which（62.86%）were polymorphic. The average number of DNA bands amplified by each primer was 11.67. There were specific amplified bands in six cultivars, and these bands can be used in Pine apple identification. The dendrogram constructed by UPGMA showed that the 17 individuals were classified into three groups. Genetic relationships of Pine apple were also discussed.
出处
《中国农学通报》
CSCD
2006年第6期428-431,共4页
Chinese Agricultural Science Bulletin
基金
科技部科技基础性工作和社会公益研究专项"热带作物种质资源DNA指纹图谱鉴定技术标准研究"(2003DIB7J066)的部分内容。
关键词
菠萝
ISSR
品种鉴定
遗传关系
Pine apple, ISSR, Cultivars identification, Genetic relationships
