摘要
取30个枣树品种进行ISSR分子标记分析,其扩增条带分别进行琼脂糖和聚丙烯酰胺凝胶电泳检测,以期获得不同产地品种之间的遗传多态性。从100条选择扩增的ISSR引物中筛选出17条扩增清晰、重复性和稳定性好的引物,选取其中8条扩增条带多态性强的引物进行遗传聚类分析。结果表明:(1)1%琼脂糖凝胶电泳和5%聚丙烯酰胺凝胶电泳检测扩增总条带数分别为72和127条,其中多态性条带分别为51条和113条,多态性条带比率(PPB)分别为70.8%和88.9%。(2)基于UPGMA软件对30个品种的遗传差异性分析表明,8个ISSR引物可以将枣树品种之间遗传差异明显区分开来。两种电泳检测方法相比较,聚丙烯酰胺凝胶电泳检测可获得较为精细的枣树品种间遗传图谱。其遗传相似系数范围在0.56～1.00之间,以0.62为最低遗传相似系数,可将30个枣树品种分成3个大类,6个亚类,为进一步研究枣树品种间分类、起源进化关系和分子辅助育种奠定基础。
Genetic diversity among 30 accessions of jujube （Ziziphusjujuba） were investigated with inter simple sequence repeats （ISSR） technique, using agarose gel electrophoresis and polyacrylamide gel electrophoresis （PAGE）. ISSR analysis was carried out 8 random primers generated from 17 polymorphic primers. The results showed that： （1） 72 and 127 DNA bands were amplified and detected by 1% agarose gel electrophoresis and 5% PAGE respectively, with the percentage of polymorphic bands （PPB） （88.9%） in PAGE detection higher than that in agarose gel electrophoresis （70.8%）; （2） An efficient separation of 30 jujube accessions was achieved using unweighted pair group method with arithmetic means （UPGMA） clustering. Those detected by PAGE electrophoresis could achieve more fine genetic map among 30 accessions of jujube compared with by agarose gel. The genetic similarity coefficient （GS） ranged from 0.56-1.00. Those detected by agarose gel electrophoresis were clustered into three groups and seven subgroups, meanwhile those detected by PAGE were clustered into three groups and six subgroups. These results indicates that ISSR analysis by PAGE is much better than that by agarose gel electrophoresis.
出处
《植物生理学报》
CAS
CSCD
北大核心
2011年第3期275-280,共6页
Plant Physiology Journal
基金
山西省留学人员科研资助项目(2010041)
山西农业大学引进人才基金(416568)
