摘要
目的探讨和比较IL-8、TNF-α、IL-1-β等细胞因子在支气管哮喘发病机制中的作用。方法哮喘急性发作期患者31例、正常对照组30例的血清和诱导痰标本,于病情缓解后作自身对照。用放免法分别测定血清和痰液中IL-8、TNF-α、IL-1-β水平。结果急性发作期血清及痰液中IL-8水平显著高于稳定期和对照组(P均<0.01);急性发作期TNF-α与缓解期比较无明显变化(P>0.05),但仍高于对照组;缓解期IL-8、TNF-α显著高于对照组(P均<0.05)。血清及痰液中IL-1-β差异无显著性(P均>0.05)。急性发作期血清中IL-8与TNF-α呈显著正相关(r=0.461,P<0.01),痰液中IL-8与TNF-α也呈显著正相关(r=0.572,P<0.01)。血清和痰液中IL-8呈显著正相关(r=0.553,P<0.01)。结论IL-8、TNF-α参与了气道炎症的形成,在哮喘的发病机制中起重要作用。
Objective To evaluate the role of cytokines in the pathogenesis Asthma. Methods The sputum and serum samples were obtained from 31 patients with attack and stable Asthma and 30 normal control. IL-8,IL-1-β,TNF-α level were measured by radioimmunoassay [RIA]. Results Compared with the control group, there was a significant increase in the level IL-8 and TNF-α in the Asthma group the sputum and serum level of IL-8 were significantly higher than those in the stable stage （P〈 0.01）. The stable stage was higher than control group （P〈0.01）, but the sputum and serum level of TNF-α were not significantly between in the attack and stable stage. The sputum and serum level of IL-1-β did not different in the three groups. In the Asthma group, the sputum and serum level of IL- 8 was correlated positively with the TNF-α （r= 0. 572,0. 461）. The sputum level of IL-8 was correla- ted positively with the serum in the Asthma （r= 0. 533）. Conclusion IL-8,TNF-α play an important role in the pathogenetic process of Asthmas.
出处
《贵州医药》
CAS
2006年第6期503-505,共3页
Guizhou Medical Journal
