摘要
目的:分析复方鸡骨草胶囊对四氯化碳、D-半乳糖胺所致小鼠急性化学性肝损伤的保护作用。方法:实验于2006-01/03在广西医科大学药学院药理学教研室实验室完成。取健康成年昆明种系小鼠144只,按随机数字表法分为12组,每组12只。分为2个实验,四氯化碳和D-半乳糖胺肝损伤实验均设6个组,正常对照组、模型组、鸡骨草胶囊组、复方鸡骨草胶囊12.96g/kg,6.48g/kg,3.24g/kg组。鸡骨草胶囊组和复方鸡骨草胶囊12.96g/kg,6.48g/kg,3.24g/kg组分别灌胃给予鸡骨草胶囊14.47g/kg和复方鸡骨草胶囊(鸡骨草胶囊的升级产品,由鸡骨草、茵陈、栀子、三七、牛黄、白芍等多种中草药配伍组成)12.96,6.48,3.24g/kg,其余各组小鼠灌胃给予等量生理盐水,灌胃1次/d,连续5d。5d后除正常对照组外,其余各组腹腔注射1g/L的四氯化碳/花生油溶液10mL/kg或腹腔注射D-半乳糖胺800mg/kg,禁食,16h后测定血清谷丙转氨酶和谷草转氨酶活性,观察肝脏组织病理学改变。结果:144只小鼠全部进入结果分析,无脱失。四氯化碳和D-半乳糖胺肝损伤2个实验中,鸡骨草胶囊组和复方鸡骨草胶囊12.96g/kg,6.48g/kg组小鼠的血清谷丙转氨酶和谷草转氨酶活性显著低于模型组(P<0.05～0.01),鸡骨草胶囊和复方鸡骨草胶囊各剂量组肝组织受损程度显著轻于模型组(P<0.05～0.01),复方鸡骨草胶囊各剂量与鸡骨草胶囊的效应比较,差异无显著性意义(P>0.05)。结论:复方鸡骨草胶囊对四氯化碳、D-半乳糖胺引起的小鼠急性化学性肝损伤均有明显的保护作用,保肝效应和鸡骨草胶囊相当。
AIM： To analyze the protective effects of compound Jigucao （CJGC） capsule against carbon tetrachloride （CCL） and D-Galactosamine （D-Gal）induced acute chemical liver injury in mice.
METHODS： The experiment was carried out in the laboratory of the Department of Pharmacology, Pharmaceutical School of Guangxi Medical University from January to March 2006. Totally 114 healthy male mice of Kunming species were randomly divided into 12 groups with 12 mice in each group. Control group, model group, Jigucao （JGC） capsule group and CJGC capsule group at the dose of 12.% g/kg, 6.48 g/kg and 3.24 g/kg were all set up in the experiments of liver injury induced by CC14 and DGal. JGC capsule, CJGC capsule or same volume normal saline were given orally to mice in corresponding groups, intragastric infusion once per day （JGC capsule group： 14.47 g/kg JGC capsule; CJGC capsule groups： 12.96 g/kg, 6.48 g/kg and 3.24 g/kg CJGC capsule, which was the upgraded products of JGC capsule and consisted of Herba Abri, Herba Artemisiae Scopariae, cape jasmine fruit, sanchi, cow-bozear and whilt poorly root, etc in compatibility）. Five days later, except the control group, the mice of other groups were administrated intraperitoneally with 1 g/L CCL/peanut oil solution （10 mL/kg） or D-Gal （800 mg/kg）. After 16-hour fasting, serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） activities were detected, and histopathological changes of liver were also observed.
RESULTS： All the 114 mice were involved in the analysis of results without drop. In the experiments of acute liver injuries induced by CC14 and D-Gal, JGC capsule and CJGC capsule at dose of 12.% g/kg and 6.48 gag remarkably decreased the activities of serum ALT and AST （compared with model group, P 〈 0.05-0.01）. Moreover, JGC and each dose of CJGC capsule notably lessened the histopathological damage （compared with model group, P 〈 0.05-0.01）. There was no significant difference in the effects of CJGC capsule and JGC capsule （P 〉 0.05）.
CONCLUSION： CJGC plays the protective effects obviously on acute chemical liver injury induced by CCl4 and D-Gal in mice, identical as JGC capsule.
出处
《中国临床康复》
CSCD
北大核心
2006年第23期142-143,共2页
Chinese Journal of Clinical Rehabilitation
