摘要
目的 探讨吲哚硒对四氯化碳 (CCl4)致小鼠急性化学性肝损伤的保护作用及可能机制。方法 建立CCl4诱导小鼠化学性肝损伤模型 ,分光光度法检测血浆中ALT、AST水平和肝匀浆MDA、SOD、GSH Px含量 ,HE染色法对肝脏组织作病理检查。结果 吲哚硒 (5、10、2 0mg/kg)灌胃给药均能降低血浆中升高的转氨酶水平 ,病理检查也发现其可明显减轻肝组织坏死范围及程度 ,减少炎细胞浸润。同时发现吲哚硒可降低肝匀浆中升高的MDA水平 ,使降低的肝匀浆SOD、GSH Px酶活性升高。结论 吲哚硒对CCl4致小鼠急性化学性肝损伤具有保护作用 ,其机制与其抗自由基、提高抗氧化物酶的活性等有关。
Objective To investigate the effects of indole-selenium on carbon tetrachloride-induced liver injury. Methods The model of mice chemical liver injury was prepared.The level of alanine aminotransferase(ALT), aspartate aminotransferase(AST) in plasma, malondiadehyde(MDA) content, superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activities in liver homogenate were assayed by spectrophotometer; Hepatic pathological examination was observed. Results Indole-selenium (5,10,20 mg/kg) was able to significantly decrease serum transaminase (ALT,AST) levels, attenuate the area and extent of necrosis and reduce the infiltration of inflammatory cells. Furthermore, indole-selenium also decreased MDA content and improved the reduced SOD and GSH-Px level in liver homogenate. Conclusion Indole-selenium possesses protective action on chemical liver injury in mice.
出处
《安徽医科大学学报》
CAS
2003年第3期179-182,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省教育厅重点科研基金资助 (编号 :98JL0 13 0Z)
