摘要
利用树研究肿瘤坏死因子ａ（ＴＮＦａ）在肝损害中的作用。结果显示，ＴＮＦａ单独或与Ｄ－氨基半乳糖（Ｄ－ＧａＩＮ）联合作用于培养树肝细胞，上清天冬氨酸氨基转移酶活性无明显变化；将ＴＮＦａ处理后的树血清作用于培养肝细胞，上清中ＡＳＴ活性升高，抑肽酶能抑制这种效应。在ＨＢｓＡｇ预先致敏的树体内注入ＴＮＦａ可导致肝、肺、肠等器官的出血、坏死。提示ＴＮＦａ在肝损害中起一定作用，并在体内外存在差异。
In this study,the effect of TNFa on liver injury was evaluated with animal experiments in vivo and in vitro. The results revealed that TNFa alone or with D-GaIN in cultured tupaia hepatocytes had no signifi- cant effect on AST activity in the supernatant,but the sera of TNF-treated tupaia could cause elevation of AST activity in cultured hepatocyte supernatant which could be depressed by aprotinin. When TNFa was injected into the animals in which HBsAg was pre-injected through portal vein,it could induce hemorrhage and necrosis in liv- er,lungs or intestine etc. These findings suggested that TNFa played a rale in liver damage,and its effect was different between in vivo and in vitro.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1996年第2期115-116,共2页
Medical Journal of Chinese People's Liberation Army
