摘要
目的：探讨成年树（Ｔｕｐａｉａ）感染ＨＣＶ的可能性。方法：用ＨＣＶＲＮＡ阳性人血清接种１０只健康成年Ｔｕｐａｉａ。观察临床表现；定期检测血清ＡＬＴ和ＨＣＶＲＮＡ正、负链；检测肝组织中ＨＣＶ３种抗原和负链ＨＣＶＲＮＡ；行肝组织病理检查。结果：Ｔｕｐａｉａ接种阳性血清后，１０例均出现不同程度的肝炎症状；１０例血清ＡＬＴ升高，其中３例在２８周时仍高于正常；６例肝组织中ＨＣＶ抗原持续阳性，持续时间大于２８周；１０例出现急性肝炎的肝组织病理学变化，其中４例有慢性肝炎病变。检测４只动物血清的系列标本，ＨＣＶＲＮＡ均呈间歇性阳性；检测３只动物肝组织负链ＨＣＶＲＮＡ均为阳性；检测１例动物肝外组织，其中心肌细胞、胰岛细胞和肾小管上皮细胞中有ＨＣＶ３种抗原染色阳性信号。结论：ＨＣＶ能够感染成年Ｔｕｐａｉａ，并可形成慢性病变，感染率约为６０％，慢性病变发生率约为４０％。
Objective: To explore the possibility of infection of hepatitis C virus (HCV) in adult Tupaiae. Methods: After 10 Tupaiae were inoculated intravenously and intraperitoneally with the human serum positive for HCV RNA, the clinical signs were observed daily and the blood and liver specimens collected regularly. The ALT levels, the positive and negative strands of HCV RNA in the serum, the HCV NS3, NS5, CP10 antigens and the negative strands of HCV RNA in the liver were examined. The hepatic morphological changes were observed with light microscope. All these steps were kept on until the 28th week after inoculation. Results: ① All the animals presented signs of hepatitis after inoculation. ② The serum ALT levels were increased and the elevated levels were detected in 3 animals even in the 28th week after inoculation. ③ The HCV antigens in the liver tissue were continuously positive in 6 animals. ④ The positive and negative strands of HCV RNA were detected intermittently in the serum of 4 animals. ⑤ In one Tupaia, the HCV NS3, NS5 and CP10 antigens were found in the cardiac muscle cells, pancreatic islet cells and renal tubular epithelial cells. ⑥ The hepatic morphological changes in all the 10 animals were similar to those in patients with acute hepatitis. Conclusion: Tupaiae can be used as the experimental animals for study on hepatitis C for they are susceptible to HCV.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1998年第6期472-475,共4页
Journal of Third Military Medical University
关键词
丙型肝炎病毒
疾病模型
树QU
hepatitis C virus
disease model/animal
Tupaia
