摘要
目的评价乙肝病毒（ＨＢＶ）实验感染树的稳定性与实用性。方法用含ＨＢＶ的２．２．１５细胞或乙肝患者混合血清以不同剂量、经不同途径接种６０只健康树。观察其临床表现，检测血清ＡＬＴ、ＨＢｓＡｇ．ＨＢｅＡｇ及ＨＢＶ－ＤＮＡ。肝组织行ＨＥ及免疫组织化学染色，结果部分树出现一过性食欲不振、稀便；６０只树不同时相ＡＬＡ水平均在正常范围；８只树ＨＢＶ标志物一过性阳性；肝组织未见类似于人乙型肝炎的典型病理改变；免疫组织化学染色为阴性。
Objective To evaluate the stability and availability of the tree shrew model with hepatitis B. Methods 60 healthy tree shrews were injected either with 2.2. 15 cells containing HBV or with confused patients serum with hepatitis B with various dose, by peritoneal injection, venous injection, and peritoneal combined with venous injection, respectively. The general clinical manifestations were observed daily. Serial blood samples were obtained and serum ALT levels, serum HBsAg, HBeAg and HRV-DNA were detected respectively. Every liver specimen obtained from 24 tree shrews was divided into two portions for immunohistochemical and light-microscopic examinations, Results Some of the tree shrews showed anepithymia, diarrhea temporally. HBV markers detection of all the experimental tree shrews were negative except 8 tree shrews in the group of venous injection, of which serum HBsAg, HBeAg and HBV-DNA were temporally positive at 2 - 3 week post injection, The livcr nonspecific morphologic changes of tree shrews were not similar to those of patients with hepatitis B. HBsAg was not detected in the hepatic cells by immunoheistochemical staining using murine monoclonal antibody against HBs antigen. Conclusion All these findings suggest that tree shrews model with hepatitis B virus was not established succeedingly. [
出处
《中华肝脏病杂志》
CAS
CSCD
1998年第2期96-97,共2页
Chinese Journal of Hepatology
