摘要
目的:探讨水通道蛋白(aquaporin,AQP)对缺血再灌注损伤后脑水肿形成过程中的影响作用,寻找防治缺血再灌注损伤后脑水肿的新途径。方法:采用完全随机对照方法,应用自行构建的含AQP4基因的真核表达质粒,在大鼠脑内进行预先转染,观察分析转染后大鼠脑内AQP4表达水平、脑卒中后脑水肿程度和大鼠脑卒中后神经功能缺损程度的变化程度,实验分正常健康组、AQP4基因干预组和基因干预对照组。结果:①单纯给予大鼠脑内注射AQP4基因24h后,大鼠的神经功能缺损评分,局部脑组织的含水量均无明显变化。②AQP4基因干预组在大鼠缺血2h再灌注3,12,24h的神经功能缺损评分分别为9.17±0.72,7.93±0.69,7.13±0.89,明显低于基因干预对照组。③再灌注12h伤侧皮质和皮质下脑水肿程度,在AQP4基因干预组分别为79.76±0.62,80.94±0.81,明显高于基因干预对照组。④脑内注射AQP4基因后可升高大鼠脑缺血再灌注损伤早期脑内AQP4的表达水平。结论:①单纯提高脑内AQP4的水平并不影响脑组织的含水量。②预先提高脑内AQP4的水平,可加速脑卒中后脑水肿形成和神经功能缺损程度。③改变脑内AQP4水平或活性可以成为新的防治脑卒中后脑水肿形成、减轻脑损伤的靶点。
AIM:To investigate the effects of aquaporin4(AQP4) on the brain edema after cerebral ischemic reperfusion and search the new method to prevent and cure the brain edema. METHODS:A completely randomized controlled experimental study was performed.The self conducted eukaryon expressed plasmid which carrying AQP4 gene,was pretransfected in the rat brain.The expressed level of AQP4,the degree of brain edema and neurological deficit scores were observed and analyzed after transfection.The rats were divided into normal healthy group(normal control group),APQ4 genetic intervention group(intervention group) and genetic intervention control group(control group). RESULTS:①Twenty four hours after injection of APQ4 gene into the rat brain,there were no obvious changes in the neurological deficit scores,volume of water content in local brain tissue.②The neurological deficit scores at 2 hour ischemia and 3,12 and 24 hour reperfusion in the intervention group(9.17±0.72, 7.93±0.69, 7.13±0.89) were obviously lower than those in the control group.③Twelve hours after reperfusion,the edema level at cortex and subcortex of the injured side in the intervention group(79.76±0.62, 80.94±0.81) were obviously higher than those in the control group.④Expression of AQP4 in the brain at the early period of cerebral ischemia reperfusion injury was increased after injection of APQ4 gene into the rat brain. CONCLUSION:①Increased local AQP4 expression in brain has no influence on the water content.②Pre expression of AQP4 accelerates the formation of cerebral edema and neuro functional injury.③Modification of AQP4 activity and regulation of AQP4 expression level will be the new strategy to prevent the cerebral edema and to reduce cerebral injury after stroke.
出处
《中国临床康复》
CSCD
2004年第28期6114-6116,共3页
Chinese Journal of Clinical Rehabilitation
