摘要
目的观察水通道蛋白4(AQP4)在脑出血(ICH)模型组及去铁胺(DFO)干预组的表达变化及作用。方法采用自体血ICH模型,应用免疫组化及RT-PCR方法观察不同组别及时间段AQP4的表达。结果AQP4阳性细胞主要分布于脑内星形细胞足突与毛细血管接触区。AQP4蛋白表达在ICH模型组及DFO干预组均较假手术组明显增高且以第7天最高,DFO干预组第7及第14天AQP4蛋白表达较同期ICH模型组显著降低。AQP4 mRNA表达在所有ICH组及DFO干预第3及第7天组较假手术组显著增高且以第3天最高,ICH组第14与第7天AQP4 mRNA表达无显著差别。DFO干预各组与同期ICH模型组相比AQP4 mRNA表达显著降低,且DFO干预各组较前一时间点AQP4 mRNA表达呈显著下降趋势,DFO干预第14天组AQP4 mRNA表达与对照组无差别。AQP4蛋白表达与AQP4 mRNA表达呈正相关关系。结论AQP4在ICH后水肿的形成及消退中可能起重要作用,DFO可能对干预ICH后水肿形成有一定作用。
Objective To study the dynamic changes of AQP4 expression between Intracerebral Haemorrhage （ICH）models and Deferoxamine （DFO） intervention models. To explore the mechanism and role of AQP4 and DFO in edema formation after ICH. Methods ICH was established by infusing autologous blood into rat putamina; Immunohistochemistry and reverse transcription polymerase chain reaction （RT-PCR） method were respectively used to evaluate AQP4 protein and mRNA expression in different group and distinct period of time. Results AQP4 positive cells mostly located in astrocyte in the perivascular glial feet processes in the brain. As compared with the sham operation groups, there appeared a significant increase of AQP4 protein expression in all ICH models and DFO intervention models; At 7 day, the AQP4 protein expression reached its maximum in ICH models and DFO intervention models; At 7 and 14 day, in DFO groups AQP4 protein expression decreased remarkably than ICH groups. There showed a significant enhanced expression of AQP4 mRNA in all ICH models and DFO intervention models at 3, 7 day compared with sham operation controls; At 3 day, AQP4 mRNA expression reached its maximum in ICH models and DFO intervention models; AQP4 mRNA expression decreased significantly in DFO models than ICH groups; There were descending trend in DFO models with the time extended; there were no differences between DFO models and controls at 14 day. There were positive correlations with AQP4 mRNA and AQP4 protein expression. Conclusions AQP4 may play a key role in edema formation and absorption after ICH, DFO was likely to be a new effective agent to intervene brain edema formation after ICH.
出处
《中国神经免疫学和神经病学杂志》
CAS
2006年第4期234-237,242,共5页
Chinese Journal of Neuroimmunology and Neurology
