摘要
目的利用高脂饮食诱发动脉粥样硬化模型,观察非诺贝特的抗动脉粥样硬化作用,并探讨其机制。方法10只新西兰大白兔给予高胆固醇饮食饲养8周后,随机分为两组:高胆固醇组继续饲以高胆固醇饲料4周;非诺贝特组在饲以高胆固醇饲料的基础上给予非诺贝特[30 mg/(kg.d)],共4周。另选择普通饮食12周兔(n=5)作为对照组。测定饲养前后血清肿瘤坏死因子α水平和饲养后的主动脉斑块面积。半定量逆转录聚合酶链反应测定脂肪组织肿瘤坏死因子αmRNA的表达。结果非诺贝特组和高胆固醇组血清总胆固醇、低密度脂蛋白胆固醇水平均明显高于对照组(P<0.001),但两组间差异无显著性。非诺贝特组与高胆固醇组相比主动脉斑块面积(52.81%±6.92%比76.30%±8.61%,P<0.01)、血管内膜厚度(28.45±5.68μm比76.18±11.25μm,P<0.05)、血清肿瘤坏死因子α水平(2.11±0.26 ng/L比3.86±0.33 ng/L,P<0.05)以及脂肪组织肿瘤坏死因子αmRNA表达量(0.31±0.05比0.56±0.07,P<0.05)均显著降低。结论非诺贝特具有一定的抗动脉粥样硬化作用,其降低高胆固醇喂养兔血清肿瘤坏死因子α水平可能是其作用机制之一。
Aim To observe the anti-atheromatous effects of fenofibrate in cholesterol-fed rabbits male New Zeahed white rabbits were fed with high cholesterol diet for 8 weeks, and then were randomly divided into two groups： high cholesterol group maintained cholesterol diet for 4 weeks; fenofibrate treatment group maintained the same cholesterol diet hosides supplementing with fenofibrate [ 30 mg/（ kg·d） ] for 4 weeks. And control group was fed with normal diet for 12 weeks. Before and after feeding, serum tumor necrosis factor-α （TNF-α ） levels of all rabbits were measured. After feeding, the aortas were harvested for observing the formation of atherosclerotic plaques. TNF-α mRNA expression in adipose tissue was evaluated by reverse transefiption-polymerase chain reaction （RT-PCR）. Results High cholesterol group and fenofibrate treatment group showed higher serum levels of total cholesterol and low density lipoprotein cholesterol than those of control group （P〈0.001 ）, but there were no difference between the two groups（P〉0.05）. As compared with the high cholesterol group, aorlic atherosclerotic area （52.8%±6.9% vs 76.3%±8.6%, P〈0.01）,intimal thickness of the aorta （28.45±5.68μm vs 76.18± 11.25μm, P〈0.05）, serum TNF-α levels （2.11±0.26 ng/L vs 3.86±0.33 ng/L, P〈0.05） and TNF-α mRNA expression in adipose tissue （0.31±0.05 vs 0.56 vs 0.07, P〈0.05 ） were significantly decreased in fenofibrate treatment group. The study indicates that fenofibrate manifests an anti-atheromatous effect independent of the hypolipidemie effect in cholesterol-fed rabbits and decreasing serum TNF-α level may be one of its possible mechanisms.
出处
《中国动脉硬化杂志》
CAS
CSCD
2006年第4期313-316,共4页
Chinese Journal of Arteriosclerosis
