摘要
目的 :观察抗血小板药物氯吡格雷对实验性动脉粥样硬化形成的影响。方法 :2 9只雄性日本大耳白兔分为正常组 (n =9)、模型组 (n =10 )和氯吡格雷组 [4mg/(kg·d) ,n =10 ]。后两组建立高胆固醇饲料并免疫损伤诱发的主动脉粥样硬化模型。测血脂和血清C 反应蛋白 (CRP)浓度。观察主动脉组织病理形态学改变并定量分析病变程度 ,免疫组化法测定斑块部位的巨噬细胞和平滑肌细胞阳性细胞百分数。结果 :与模型组相比 ,氯吡格雷组的主动脉斑块面积 /内膜面积减少 2 7 5 2 % ,内膜厚度和内膜厚度 /中膜厚度分别减少 5 4 5 2 %和 5 0 98% ,斑块中巨噬细胞百分数从 ( 5 7 5 0± 8 40 ) %减至 ( 4 0 93± 7 0 8) % ,平滑肌细胞百分数从( 3 7 2 8± 3 3 5 ) %增至 ( 5 3 16± 6 96) % ,血清CRP浓度从 ( 2 67 86± 49 89)ng/ml降至 ( 189 82± 77 0 2 )ng/ml,均有显著性差异 (P <0 0 5～ 0 0 1)。结论 :氯吡格雷能够减轻动脉炎症反应 ,抑制动脉粥样硬化的形成。
Objective: To observe the effects of clopidogrel on the formation of experimental atherosclerosis. Methods: Twenty-nine male rabbits were divided into three groups: normal group ( n =9),model group( n =10) and clopidogrel group 4 mg/(kg·d)( n =10). Rabbits of last two groups were fed on 2% cholesterol diet for 12 weeks and were injected with 10% Albumin Bovine. Serum lipids and C-reactive protein (CRP) were detected. The aortas were harvested for histomorphometric observation and quantitative analysis. The positive percentage of smooth muscle cells and macrophage cells was detected by immunohistochemistry. Results: Compared with model group,clopidogrel group showed that the ratio of lesion to intimal area decreased by 27.52%,intimal thickening and intima-to-media ratio respectively reduced by 54.52% and 50.98%,the positive percentage of macrophage cells decreased from 57.50±8.40% to 40.93±7.08%,the positive percentage of smooth muscle cells increased from 37.28±3.35% to 53.16±6.96%,and concentration of serum CRP decreased from 267.86±49.89 ng/ml to 189.82±77.02 ng/ml( p <0.05—0.01). Conclusion: Clopidogrel can suppress vascular inflammation and inhibit the formation of atherosclerosis.
出处
《中国循环杂志》
CSCD
北大核心
2004年第2期145-148,共4页
Chinese Circulation Journal
