摘要
目的探讨十全大补汤对H22肝癌小鼠免疫功能的影响,为十全大补汤在临床上的应用提供理论依据。方法60只昆明种小鼠(雌雄各半)随机分为6组,分别灌服生理盐水及低、中、高浓度的十全大补汤,腹腔注射5-氟尿嘧啶(5-FU),10d后颈椎脱臼处死。用鸡红细胞法检测H22肝癌小鼠腹腔巨噬细胞(Mφ)吞噬功能;3H-TdR掺入法检测T淋巴细胞转化功能;采用YAC-1为靶细胞的3H-TdR前标法进行NK细胞活性测定。结果十全大补汤中剂量组、高剂量组抑瘤率与模型组比较,差异显著(P<0.01);与正常对照组比较,模型组、5-FU组T淋巴细胞转化功能、NK细胞活性和Mφ吞噬百分率均明显下降,差异显著(P<0.01);十全大补汤高、中剂量组T淋巴细胞转化功能、NK细胞活性与正常对照组比较无显著差异(P>0.05);与模型组比较,各组小鼠的Mφ吞噬率无显著差异(P>0.05)。结论十全大补汤可提高机体的免疫功能,抑制肿瘤的生长。
Objective To investigate the influence of All Nourishing Decoction on immunity of mice with H22 liver cancer, which will provide theoretical evidence for clinical diagnosis and treatment. Methods Divided 60 mice （female equal male） into 6 groups randomly, fed on saline solution, low dose, moderate dose and high dose of All Nourishing Decoction, 5-FU injection respectively. Kill the mice by breaking C-spine after 10 days. The method of enveloping CRBC was adopted to explore the function of macrophage in abdominal cavity. 3H-TdR marking method was used to observe the proliferation of T lymphocytes and the method of aH-TdR signal which use YAC-1 as TC was applied to measure the activity of NK cell. Results Lump-inhibition rates of moderate and high dose group of All Nourishing Decoction were remarkable higher than that of model group （P〈0.01）. The function of T-lymphocytes and macrophage, the NK cell activity of model group and 5-FU group is noticeable lower than that of normal group, while among the high, moderate dose group of All Nourishing Decoction, and normal group, there was no significant difference （P〉0.05）. Comparing the function of macrophage of all groups with that of the model group, it had not remarkable difference （P〉0.05）. Concluion All Nourishing Decoction can enhance the immunity of the＇body, inhibit the growth of tumor.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第6期33-34,共2页
Chinese Journal of Information on Traditional Chinese Medicine
