摘要
目的:观察联合应用重组人粒细胞集落刺激因子(rhG-CSF)和干细胞因子(rhSCF)对大鼠急性心肌梗死(AMI)心室功能、梗死面积及心室重塑的影响。方法:采用异丙基肾上腺素法将32只雄性SD大鼠制为AMI模型,3h后按皮下注射药物类别随机分为4组,A组为rhG-CSF与rhSCF合用组,B组为rhG-CSF组,C组为 rhSCF组,D组为对照组。每组8只,各组随机分为2个亚组,分别于14d和28d观察心室功能后处死,取出心脏,称重比较心室重塑差异,HE染色,体视学方法观察梗死面积大小及药物治疗作用。结果:(1)给药14d及28d后,A组心功能优于B、C、 D组(P<0．05);左心室质量／体质量(g/G)小于C、D组(P<0．05);梗死面积／左心室总面积(s／S)小于B、C、D组(P<0．05)。(2)A组28 d亚组较14 d亚组心功能提高, 梗死面积减小(P<0．05);心室重塑改善不明显(P>0．05)。B、C、D组的亚组间各指标无明显差异。结论:rhG-CSF和rhSCF合用对AMI大鼠缺血损伤心肌的保护和再生作用优于单用,可明显改善AMI大鼠的心室功能。
Objective： To investigate the effect of combined rhG-CSF and rhSCF mobilization on bone-marrow stem cells for improving cardiac function, infarct size and ventricular remodeling in acute myocardial infarction rats. Methods：Three hours after injecting isoprenaline to develop acute myocardial infarction model. 32 Sprague-Dawley rats were randomly allocated to rhG-CSF ＋ rhSCF （group A, n=8）, rhG-CSF （group B, n=8）, rhSCF（group C, n=8） or control treatment （group D, n=8）. After 2 to 4 weeks, the parameters of left ventricle systolic function （LVSP,LVEDP and dp/dt） were assessed by echocardiography and the g/G, s/S were measured, respectively. Results： After treatment for 2 and 4 weeks, group A had greater LVSP, LVEDP and dp/dt but smaller s/S than groups B,C,D （P〈0.05）, group A had lower g/G than C,D groups （P〈0.05）. Comparing with treatment for 2 weeks, cardiac function was improved significantly and infarct size was reduced markedly in group A for 4 weeks, but the ventricular remodeling was no significantly changed. No significant difference was found in groups B.C.D. Conclusions： Combination of rhCrCSF and rh- SCF provides significant improvements in cardiac function than single use of each agent in the treatment of acute myocardial infarction.
出处
《国际心血管病杂志》
2006年第2期129-132,共4页
International Journal of Cardiovascular Disease
关键词
急性心肌梗死
粒细胞集落刺激因子
干细胞因子
Acute myocardial infaretion
Granulocyte colony-stlmulatlng factor
Stem cell factor
