摘要
以金钱树[Zamioculcaszamiifolia(Loddiges)Engler]的幼嫩小叶为外植体,采用幼叶→愈伤组织→丛生芽→完整植株的途径进行繁殖。结果表明:叶片在MS+BA2mg/L+2,4-D1.0mg/L+PVP0.5mg/L+活性炭0.5mg/L+蔗糖30g/L培养基上暗培养30d,可诱导形成愈伤组织;愈伤组织在MS+BA4mg/L+KT0.5mg/L+蔗糖30g/L培养基上光照培养25d,可诱导形成丛生芽(丛生芽在继代培养过程中每20～25d可增殖3～5倍);丛生芽接种于MS+BA3mg/L+NAA0.2mg/L+蔗糖30g/L培养基上壮苗培养4周后,再转入1/2MS+BA2mg/L+NAA0.5mg/L+蔗糖30g/L培养基上培养30d,可诱导形成完整的根系。
Leaf explants of Zamioculcas zamiifolia （Loddiges） Engler were inoculated on MS medium containing 2 mg/L BA （6-benzylaminopurine）, 2,4-D （2,4-dichlorophenoxya-cetic acid） 1.0 mg/L, PVP （Polyvinylpyrolidone） 0.5 mg/L, AC （Active carbon） 0.5 mg/L, 30 g/L Suc for inducing callus. After being cultured in darkness for 25 days, calli were formed. They were then transferred onto the MS medium supplemented with 4 mg/L BA, 0.5 mg/L KT and 30 g/L Suc for inducing clustered buds. The clustered buds were formed after being cultured for 25 days （they were multiplied 3-5 times every 20-25 days of subculture）. After cultured on the MS medium containing 0.2 mg/L NAA, 3 mg/L BA and 20 g/L Suc for about 4 weeks, the clustered buds were transferred onto the 1/2 MS medium containing 0.5 mg/L NAA, 2 mg/L BA and 30 g/L Suc for roofing. This cultural system is very useful for commercial proliferation of Z. zamiifolia.
出处
《热带农业科学》
2006年第2期19-21,32,共4页
Chinese Journal of Tropical Agriculture
