摘要
作者运用生化和分子生物学方法，探讨了抗肝炎药联苯双酯（ＤＤＢ）对人肝癌细胞分化的影响，结果发现：生长在含有１０－４ｍｏｌ／ＬＤＤＢ培养液中的人肝癌Ｂｅｌ－７４０２细胞，其甲胎蛋白（ＡＦＰ）的分泌量和γ－谷氨酰转肽酶（γ－ＧＴ）活力均明显低于培养相同天数的对照细胞，而白蛋白（ＡＬＢ）的分泌量和酪氨酸－α酮戊二酸转氨酶（ＴＡＴ）活性又明显高于对照细胞。ＤＤＢ还能明显增加Ｂｅｌ／７４０２细胞环化腺苷酸（ｃＡＭＰ）的含量，并抑制癌基因ｃ－ｍｙｃ及肝癌标志ＡＦＰ基因的表达，增强了Ｐ５３抑癌基因的表达。上述结果提示ＤＤＢ能诱导人Ｂｅｌ－７４０２肝癌细胞的分化。
When human Bel-7402 hepatocarcinoma cell line grew in a medium containing 10-4M DDB,the secretion of α-fetoprotein(AFP) and the activity of γ-glutamyltranspeptidase(γ-GT)were significantly lower than the control cells,whereas the albumin(ALB) secretion and the activity of tyrosine-α-ketoglutarate transaminase(TAT)were markedly increased.DDB at the concentration of 10-4M could significantly increase the content of cAMP in Bel-7402 cells,and also suppressed the expressions of oncogene c-myc and hepatocarcinoma marker AFP gene and enhanced the anti-oncogene p53 expression.The results of this paper suggest that DDB has some reversing effects on the phenotypes of human Bel7402 hepatocarcinoma cell line.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1995年第11期676-678,共3页
National Medical Journal of China
