摘要
目的观察苦荞壳提取物对高脂饲料诱导的大鼠脂肪肝的预防作用,探讨其机制.方法雄性Wistar大鼠50只,按体质量随机分为普通饲料组、高脂模型组、苦荞壳提取物高剂量组(0.825g/kg)、低剂量组(0.165g/kg)和力平之对照组(0.067g/kg).于实验后第12wk测定大鼠肝脏Ch,Tg,MDA浓度和SOD,GPX活力,并观察大鼠肝组织病理变化.结果高脂模型组大鼠肝脏Ch,Tg,MDA含量显著高于普通饲料组(P<0.01);SOD活力显著低于普通饲料组(P<0.01);GPX活力低于普通饲料组,但无统计学差异(P>0.05).与高血脂模型组相比,苦荞壳提取物各组大鼠肝组织Ch浓度显著降低,SOD活力显著升高(P<0.05),GPX活力升高,但无统计学差异(P>0.05);高剂量组Tg,MDA浓度显著降低(P<0.05),低剂量组Tg,MDA浓度降低,但无统计学差异(P>0.05).力平之组大鼠肝脏Ch降低(P<0.05),Tg,MDA含量和SOD活力无显著性变化(P>0.05);而GPX活力显著降低(P<0.05).结论苦荞壳提取物可减少高脂血症大鼠肝脏脂质沉积,降低肝脏脂质过氧化水平,提高肝脏抗氧化能力,预防脂肪肝的形成.
AIM： To investigate the preventive effect of tartarian buckwheat shell extract （TBSE） on fatty liver induced by highlipid diet in rats and it mechanism. METHODS： Fifty male Wistar rats were randomly separated into 5 groups according to body weight. They were fed with normal diet, high-lipid diet, high-lipid diet supplemented with different concentrations of TBSE （0. 825 g/kg, 0. 165 g/kg）, high-lipid diet supplemented with Lipingzld capsules （LPZ, 0. 067 g/kg）, respectively. After 12 weeks, in liver tissues, Ch, Tg, MDA concentrations, SOD, GPX activities were measured, and the histological changes were observed. RESULTS： Compared with normal diet group, in high-lipid diet group liver Ch, Tg, MDA concentrations increased significantly （ P 〈 0. 01 ） ; liver SOD activity decreased significantly （P 〈0.01 ）; liver GPX activity decreased, but there was no significantly statistical difference （ P 〉 0.05 ）. Compared with high lipid diet group, in TBSE groups, liver Ch concentration decreased significantly （P 〈 0.05 ）, liver SOD activity increased significantly （P 〈 0.05 ）, and liver GPX activity increased, but there was no significantly statistical difference （ P 〉 0.05 ） ; in high-dose TBSE group, liver Tg, MDA concentration decreased significantly （ P 〈 0. 05 ） ; in low-dose TBSE group, liver Tg, MDA concentration decreased, but there was no significantly statistical difference （ P 〉 0. 05 ）. In LPZ group, compared with high-lipid diet group, liver Ch concentration, GPX activity decreased significantly （ P 〈 0.05 ） ; liver MDA, Tg concentration, SOD activity had no significantly statistical changes （ P 〉 0.05 ）. CONCLUSION： TBSE could decrease liver Ch, Tg concentration, reduce liver lipid deposition and lipid peroxidation, increase liver antioxidant activity in rats with hyperlipidemia, which could protect hepatocytes from fatty degeneration.
出处
《第四军医大学学报》
北大核心
2006年第10期883-885,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目(30270350)
关键词
苦荞壳提取物
脂肪肝
大鼠
抗氧化剂
tartarian buckwheat shell extract
fatty liver
rats
antioxidants
