摘要
用细胞电穿孔融合仪，摸索最佳电融合参数，对５种分枝杆菌超声粉碎菌体混合抗原免疫的１只ＢＡＬＢ／Ｃ小鼠脾细胞和ＮＳｌ骨髓瘤细胞进行了电融合。在最佳电融合参数下，获得１．７５个杂交瘤细胞克隆／１０５个脾细胞的最佳融合率。采用间接ＥＬＩＳＡ法筛选，经克隆化，获２２株分泌抗分枝杆菌单克隆抗体（ＭｃＡｂ）的阳性杂交瘤细胞株。并对其中１１株进行了初步研究。
By using the electrofusion technique,the fusion of NSl myelona cells with spleen cells of BALB/c mouse immunized with the five species of mycobacteria was carried out.With the optimal electrofusion conditions,we obtained a high fusion rate of l.75 hybrid cell clones per l05 spleen cells.ELISA was used for screening secreting anti-mycobacteria McAb hybrid cells.After cloning,22 stable hybrid cells lines were selected,and 11 of them had been studied.
出处
《天津医科大学学报》
1996年第2期23-26,共4页
Journal of Tianjin Medical University
关键词
单克隆抗体
分枝杆菌
电融合
杂交瘤
Cell electrofusion
monorclonal antibody
mycobateria
ELISA
