摘要
将１×１０７个经肠道腺病毒（Ａｄ４０／４１）免疫的小鼠脾细胞与３×１０６个骨髓瘤细胞进行电融合，再置ＩＭＤＭ甲基纤维素半固体选择培养基中进行选择培养，一次性得到１８个杂交瘤细胞克隆，并从中筛查出７株高阳性抗体分泌克隆。从而成功地运用电融合与半固体培养相结合的方法以少量的材料制得了抗肠道腺病毒单克隆抗体。
Ten million splenocytes from the enteric adenovirus (Ad40/41) immunized mice were fused with 3×10 6 myeloma cells by electrofusion. Then, the cells were selectively cultured in methyl cellulose semi-solid medium. Eighteen clones of hybridoma were directely picked out from the culture plates and 7 of them could secret high level specific antibodies to enteric adenovirus. So far, the anti-Ad40/41 virus monoclonal antibodies were successfully generated by the protocal of electrofusion accompanied with selective culture in methyl cellulose semi-solid medium.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1997年第1期69-71,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
电融合
半固体培养
肠道腺病毒
单克隆抗体
制备
Electrofusion
Methyl cellulose semi-solid medium
Enteric adenovirus
Monoclonal antibodies
