摘要
目的检测骨桥蛋白(osteopontin protein,OPN)mRNA在含钙化灶乳腺组织中的表达,及其在乳腺癌转移发生发展中的意义。方法采用RT-PCR法检测1 2 8例乳腺病变组织及相应癌旁乳腺组织和9例乳癌转移淋巴结中骨桥蛋白mRNA的表达。结果骨桥蛋白mRNA表达在含钙化灶乳腺癌组织及转移淋巴结组织中表达最高,良性钙化灶乳腺组织次之,而在癌旁乳腺组织及良性无钙化病变组织中表达最低,差异有显著意义(P<0.0 5)。结论骨桥蛋白的表达与乳腺癌组织钙化有关,并可能与乳腺癌的发生有关,检测其表达水平,可望能指导临床治疗,评判患者预后。
Objective To investigate the expression of osteopontin （ OPN ） mRNA in breast tissues containing microcalcifications and the significance of OPN in tumor pathogenesis and metastasis of breast cancer. Methods The expression of OPN mRNA in 12 g samples of tissues of breast lesions and adjacent breast tissues and 9 samples of metastatic lymph nodes were examined. Results The expression of OPN mRNA was highest in calcified foci of breast cancer tissues and in metastatic lynph nodes, lower in calcified foci of benign breast tissues and lowest in breast tissues adjacent to breast cancer and in benign tissues without calcification. The differences between the defferent tissues were significant （ all P 〈0. 05 ） . Conclusions Expression of OPN mRNA is related to calcification of breast cancer tissue and to the development of breast cancer. Determination of OPN mRNA expression can be expected to be a guide to clinical therapy and prediction of the prognosis of breast cancer patients.
出处
《中国普通外科杂志》
CAS
CSCD
2006年第4期250-252,共3页
China Journal of General Surgery
关键词
乳房肿瘤
病因学
钙化
病理学
骨桥蛋白
Breast Neoplasms/etiol
Calcification, Pathologic
Osteopontin
