摘要
目的：研究汉族妇女中p21codon31单核苷酸多态性与宫颈癌易感性之间的关系.方法：用DNA抽提试剂盒从研究对象的外周血标本中抽提基因组DNA,其中宫颈癌患者226例（鳞状细胞癌215例,宫颈腺癌11例）,正常对照组196例;用错配扩增突变检测PCR的方法测定p21codon31单核苷酸多态基因型.结果：宫颈鳞状细胞癌患者的p21codon31AGA（精氨酸）等位基因频率显著高于对照组（37.0%vs 24.0%,P＜0.05,OR=1.9,95%CI=1.0～3.4）;宫颈鳞状细胞癌与对照组之间的AGA/AGA、AGA/AGC和AGC/AGC等位基因型的分布差异有统计学意义,其中AGA/AGA（OR=2.5,95%CI=1.4～4.5）和AGA/AGC（OR=1.8,95%CI=1.6～2.8）等位基因型在宫颈鳞状细胞癌中的频率显著高于对照组.宫颈腺癌与对照组之间p21codon31单核苷酸多态性分布没有显著差异.结论：p21codon31AGA（精氨酸）等位基因可能是汉族妇女患宫颈鳞状细胞癌的一个危险因素.
Objective： To investigate association between the genotype distribution of p21 codon 31 single nucleotide polymorphisms and cervical cancer in Chinese Han women. Methods：Peripheral blood samples were obtained from patients with cervical cancer（ n = 226, 215 squamous cell carcinomas and 11 adenocarcinomas）and healthy controls （n = 196 ）. DNA extracting kit was used for genomic DNA preparation, and the mismatch amplification mutation assay-PCR（MAMA-PCR） was used to detect the biallelic polymorphisms at codon 31 of p21 gene. Results： The AGA （Arg） allele frequency in patients with squamous cell carcinoma （SCC） was significantly higher than that in controls（37.0% vs 24.0% ,P 〈0.05 ,OR = 1.9, 95% CI = 1.0～3.4）. The distribution of AGA/AGA, AGA/AGC, and AGC/AGC genotpyes was significantly different between SCC patients and controls （ P 〈 0.05 ）, AGA/AGA （ OR = 2. 5,95% CI = 1.4 ～ 4.5 ） and AGA/AGC （ OR = 1.8,95% CI = 1.6 ～ 2.8 ） genotypes were more frequently found in squamous cell cancer than those in controls. There was no significant different in genotype distribution between cervical adenocarcinomas and controls. Conclusion： p21 codon 31 with AGA （Arg） allele may be a risk factor of squamous cervical carcinoma in Chinese Han women.
出处
《现代妇产科进展》
CSCD
北大核心
2006年第4期269-271,275,共4页
Progress in Obstetrics and Gynecology
关键词
宫颈肿瘤
基因
p21
多态性
单核苷酸
Cervix neoplasms
Genes, p21
Polymorphism, single nucleotide
