摘要
本文分别以自制抗-OTA兔血清抗体(IgG)及鸡卵黄抗体(IgY)就影响检测OTA的ELISA(酶联免疫吸附法)体系因素进行了探讨,建立了检测OTA的ELISA方法。鸡卵黄抗体的最佳ELISA操作参数为:包被抗原浓度为7.5μg/ml,封阻剂浓度为2.5%,抗体稀释倍数为1000,酶标二抗稀释倍数为14000;兔血清抗体的最佳ELISA操作参数为:包被抗原浓度为2.5μg/ml,封阻剂浓度为2.5%,抗体稀释倍数为8000,酶标二抗稀释倍数为10000。IgG和IgY的检测灵敏度分别为10ng/ml和1ng/ml。
In the paper, the methods of the indirect competitive ELISA for determining OTA were developed by using self- prepared rabbit serum antibody （IgG） and immunoglublin yolk （IgY）. The optimum ELISA parameters with IgY were that the concentration of antigen and blocking agent was 7.5μg/ml, 2.5% and the dilution multiples of IgY and anti-chicken rabbit antibody-HRP were 1000 and 14000, respectively. The optimum parameters with rabbit IgG were that the concentration of antigen and blockaded agent was 2.5μg/ml, 2.5% and the dilution multiples of rabbit IgG and anti-rabbit goat antibody-HRP were 8000 and 10000, respectively. The detection limits of the ELISA with IgY and rabbit IgG were 10ng/ml and 1ng/ml, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第5期30-35,共6页
Food Science
基金
江西省科技厅农业重点课题(200315A)
