摘要
目的:建立同时测定骨健口服液中3种水溶性成分—丹参素、原儿茶醛和丹酚酸 B 含量的分析方法。方法:高效液相色谱梯度洗脱法。色谱柱:Zorbax Eclipse SB-C_(18)(4.6 mm×150 mm,5 μm);流动相:0.05%(v/v)磷酸水溶液(A)-乙腈(B)梯度洗脱;检测波长:280 nm;流速:1.0 mL·min^(-1);柱温:25℃;进样量为20 μL。结果:丹参素、原儿茶醛和丹酚酸 B 的线性范围分别在0.375～7.5 μg、0.025～0.5 μg、0.966～19.32 μg,线性关系良好(r≥0.9998)。平均加样回收率(n=5)分别为99.2%,97.6%,98.9%;RSD<2.0%。结论:本方法操作简便、灵敏度高、重现性好,为全面控制和评价骨健口服液制剂的质量提供了可靠的方法。
Objective：To develop an RP- HPLC method for simultaneous determination of 3 water- soluble components -danshensu, protocatechuic aldehyde and salvianolic acid B in Gujian oral liquid. Methods：The analysis was carried on a column of RP - C18 （ Zorbax Eclipse SB - C18 ,4. 6 mm 150 mm, 5μm） by HPLC at 25 ℃, with gradient elution of 0. 05 % （v/v） phosphoric acid （A） - acetonitrile （ B ） as the mobile phase ; The flow rate was 1.0 mL · min^-1. The detection wavelength was 280 nm, and the sample injection volume was 20 μL. Results：The linear ranges of danshensu, protocatechuic aldehyde and salvianolic acid B were 0. 375 - 7.5μg, 0. 025 - 0. 5 μg and 0. 966 -19. 32μg respectively,and all the compounds showed good linearity（ r≥0. 9998 ） in corresponding detected concentration range. The average recoveries were 99. 2% ,97.6% and 98.9% ,RSD were less than 2. 0%. Condusion ： The method is simple, sensitive, accurate, reproducible, and can be used to control the quality of Gujian oral liquid.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第4期519-522,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
骨健口服液
丹参素
原儿茶醛
丹酚酸B
高效液相色谱法
梯度洗脱
含量测定
Gujian oral liquid
danshensu
protocatechuic aldehyde
salvianolic acid B
HPLC
gradient elution
quantitative determination
