摘要
目的:采用 RP-HPLC 法测定消银片中丹皮酚和芍药苷的含量。方法:采用十八烷基硅烷键合硅胶为固定相。丹皮酚的测定,以乙腈-0.1%三乙胺溶液(用磷酸调 pH=6)(55:45)为流动相,流速:1 mL·min^(-1),检测波长为274 nm;芍药苷的测定,以乙腈-水(25:75,pH=7)为流动相,流速:1 mL·min^(-1),检测波长为229 nm。结果:丹皮酚和芍药苷的线性范围分别为0.125～5μg·mL^(-1)和5～80μg·mL^(-1)。高、中、低3种浓度下的平均加样回收率,丹皮酚分别为:100.6%,102.6%,97.33%,RSD:0.52%,0.45%,0.73%;芍药苷分别为:100.1%,100.2%,98.57%,RSD:0.89%,1.2%,1.2%。结论:本方法简便,灵敏,重现性好。
Objective：To establish an RP - HPLC for determination of the content of paeonol and paeoniflorin in Xiaoyin pills. Methods：Both paeonol and paeoniflorin were separated on an RP C18 column. The determination of paeonol with acetonitrile - 0. 1% triethylamine （ adjusted to pH 6 with phosphoric acid） （ 55 ： 45 ） as the mobile phase, The flow rate was 1 mL . rain-land the detection wavelength was 274 nm;The determination of paeoniflorin with acetonitrile-water（25： 75,pH =7） as the mobile phase. The flow rate was 1 mL·min^-1 and the detection wavelength was 229 nm. Results：The linear ranges were 0. 125 -5 μg ·min^-1 for paeonol and 5-80 μg ·min^-1 for paeoniflorin. The mean recovery of high, middle, low concentration was 100. 6%, 102. 6% ,97.33% with corresponding RSD of 0. 52% ,0. 45% ,0. 73% for paeonol and 100. 1% ,100. 2% ,98.57% with corresponding RSD of 0. 89%, 1.2%, 1.2% for paeoniflorin. Conclusion： The method is simple, sensitive and reproducible.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第4期460-462,共3页
Chinese Journal of Pharmaceutical Analysis
