摘要
目的建立大鼠血浆中灯盏花乙素的HPLC-ECD测定方法,研究大鼠静注及灌胃给药灯盏花乙素的药物动力学。方法色谱柱Kromasil C_(18)柱(4.6 mm×150 mm,5μm)；流动相pH2.6的50 mmol·L^(-1)磷酸盐缓冲液-甲醇-四氢呋喃(60：40：10)；流速1 mL·min^(-1)；分析电压100 mV。结果灌胃给药后,约在1.0 h,血药浓度达第一个高峰,约在5.0 h,血药浓度达第二个峰。结论灯盏花乙素静注给药的药-时曲线符合三室模型,经剂量校正,灌胃给药的绝对生物利用度为2.20%。
OBJECTIVE To develop a HPLC - ECD method for determining scutellarin in rat plasma anti to study the phannaeokinetics of seutellarin in rats. METHODS The analytical column was Kromasil C18 （4.6 mm × 250 mm, 5 um）, and the mobile phase consisted of methanol, 50 mmol·L^-1 phosphate buffer（adjusted by phosphoric acid to pH2.6）, anti tetrahydroturan（40 ： 60 ： 10）, the flow rat was 1.0 mL·min^-1, the potential electrode voltage was 100 mV. RESULTS After oral administration of scutellarin to rats at the doses of 34 mg·kg^-1, the plasma concentration of scutellarin reached its first peak in 1.0 h, with the second peak in about 5.0 h. The concentration-time curve of scutellarin and the main pharmaeokinetic parameters were obtained. CONCLUSION The phannacokinetic behavior of scutellarin iv belongs to the three-compartment model.The absolute bioavailability of scutellarin for oral administration was 2.20%.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第9期689-692,共4页
Chinese Pharmaceutical Journal
