摘要
目的研究铅对小鼠学习记忆及自主活动的影响。方法将经过行为学筛选的48只2月龄昆明小鼠随机分成4组,醋酸铅组(简称成年组)、生理盐水组、假手术组及无处理组,每组12只,雌雄各半;将12只2周龄昆明小鼠设为幼年组。成年组及幼年组脑室内注射1μl1mol/L醋酸铅,生理盐水组注射生理盐水1μl,假手术组不注射,无处理组不进行任何干预。于脑室内注射后3d、5d、1周、2周及3周运用程控自主活动箱及LashleyⅢ水迷宫检测小鼠活动度和学习记忆能力的变化。结果成年组水迷宫潜伏期明显延长,成年组及幼年组3d～3周水迷宫潜伏期分别与生理盐水组、假手术组及无处理组比较,差异均有统计学意义(均P<0.01)。成年组5d及1周自主活动次数增加,与生理盐水及假手术组比较,差异均有统计学意义(均P<0.05),幼年组5d、1周及2周自主活动次数增加,与生理盐水及假手术组比较,差异均有统计学意义(P<0.05或P<0.01)。结论脑室内注射醋酸铅能明显降低小鼠的学习记忆功能,增加自主活动次数。
Objective To study the neurotoxicity of lead to mice and the mechanism. Methods Two months old（grown） and two weeks old（infancy） mice were employed, 1μl of 1 moL/L lead acetate was given by brain ventricle injection, 3 d, 5 d, 1 week, 2 weeks and 3 weeks after treatment with lead, the change of autonomic activities and the ability of study and memory of the mice were examined. Results The latency period of Lashley Ⅲ water maze prolonged significantly in grown group and the infancy group compared with the control groups at every time point of test. Conclusion Lead exposure can damage the function of study and memory of mice, and increase the frequency of autonomic activities of theirs.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第3期237-239,共3页
Journal of Environment and Health
基金
安徽省教委自然科学基金资助项目(2003kj226)
关键词
铅中毒
神经系统
迷宫学习
记忆
Lead poisoning, nervous system
Maze learning
Memory
