摘要
目的研究一种新型酶电极电化学性能,该电极能够被应用到生物燃料电池中。方法通过生物素-亲和素系统固定葡萄糖氧化酶,制备了生物燃料电池酶电极,应用循环伏安法研究了酶电极的电化学性能,并讨论了酶的层数、底物浓度、扫描速率和温度等条件对其性能的影响。结果通过生物素、亲和素间的特异性结合力固定化酶制备的生物燃料电池酶电极对葡萄糖有较好的电流响应。结论制备的酶电极能够满足生物燃料电池的要求,适合在人体环境中使用。
Objective To study the electrochemical features of an improved enzyme bioelectrode for biofuel cell. Methods The enzyme electrode was prepared via biotin-avidin technology and its electrochemical features were explored. The conditions such as layers of enzyme films, strength of glucose, scan speed and temperature, which affected the electrochemical behavior of enzyme electrode, were studied. Results The results showed that the electrode had good electric current response to glucose. The enzyme electrode prepared can fulfill the need of biofuel cell and is a promising electrode to be used in human body.
出处
《国际生物医学工程杂志》
CAS
2006年第2期65-68,共4页
International Journal of Biomedical Engineering
关键词
生物燃料电池
酶电极
电化学
生物素-亲和素
biofuel cell
enzyme electrode
electrochemical behavior
biotin-avidin
