摘要
采用葡萄糖氧化酶(GOD)为模板,利用"Click"反应固定,构建了新型葡萄糖生物传感器。首先,将碳纳米管叠氮化,并将GOD炔基化;在Cu^+的催化作用下,两者发生"Click"反应;在Nafion的作用下固定在玻碳电极表面,制得葡萄糖生物传感器。采用傅里叶红外光谱(FTIR)法对碳纳米管"Click"反应前后的性质进行了表征。采用循环伏安法和计时电流法考察了电极的电化学行为,并对传感器的性能进行了详细研究。结果表明:在优化的实验条件下,此电极对葡萄糖有明显的催化作用,电流与葡萄糖的浓度在6.0×10-7～1.4×10-3 mol/L范围内呈现良好的线性关系,检出限达2.0×10^(-7)mol/L。此传感器具有良好的灵敏度、稳定性和重现性。对血清样品中的葡萄糖进行检测,结果令人满意。
A novel immobilized method,＂click＂ reaction,was employed to capture glucose oxidase （GOD）.First,azido-terminated carbon nanotubes（CNTs-N_3） were obtained by bifunctional azide molecule（amine-PEG-azide） and alkynyl-modified GOD（alkynyl-GOD） was also synthesized.Subsequently, CNTs-N_3 was reacted with alkynyl-GOD in a copper（I）-catalyzed click reaction.Finally,the mixture was dipped onto the surface of glassy carbon electrode with the help of Nafion to construct a highly sensitive biosensor for the detection of glucose.The results of the both click reaction were monitored by IR spectroscopy,and the electrochemical performance of the modified electrode was studied by cyclic voltammetry and chronoamperometry.Under optimal conditions,the developed sensor exhibited fast response to glucose and the linear ranges were 6.0×10^（-7）—1.4×10^（-3）mol/L with a detection limit of 2.0×10^（-7） mol/L（at 3σ）.The proposed biosensor exhibited high sensitivity,longterm stability,excellent reproducibility.In addition,the serum samples were analyzed by this biosensor with satisfactory results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2012年第11期1642-1647,共6页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.21205146)
重庆市自然科学基金(No CSTC 2009BA5083)资助项目
