摘要
采用高产酿酒酵母(Saccharomyces cerevisiae)SCY6发酵葡萄糖产乙醇,设计单因素试验考察该酵母菌株适宜的生长条件,采用正交试验优化酵母发酵产乙醇的条件。结果表明,该酵母菌株的最适生长温度和pH分别为28℃、5.0,培养基中葡萄糖质量分数为15%时其生长状态较好。正交试验结果表明,最适合该酿酒酵母发酵产乙醇的条件为玉米浆和(NH4)2SO4作为氮源,用量分别为20 g/L和2 g/L,接种量为4%,pH 5.0。在此条件下进行发酵,发酵液中乙醇体积分数可达7.77%,葡萄糖转化率达83.82%。
The high ethanol-producing Saccharomyces cerevisiae strain SCY6 was used to ferment glucose to ethanol.Single factor tests were conducted to optimize the cultivation conditions;while orthogonal design was adopted to optimize ethanol fermentation conditions.The results showed that the optimum temperature and pH for yeast growth was 28 ℃ and 5.0,respectively.The yeast grew well when mass ratio of glucose in YPD medium was 15%.The result of orthogonal test showed that the optimal ethanol fermentation conditions were,2 g/L（NH4）2SO4 and 20 g/L corn syrup as N source;inoculation dose,4% volume fraction;and pH 5.0.The yield of ethanol reached 7.77%;and the conversion rate of glucose was 83.82% under these conditions.
出处
《湖北农业科学》
北大核心
2012年第21期4856-4859,共4页
Hubei Agricultural Sciences
基金
国家林业局引进国际先进林业科学技术项目(2009-4-63)
江苏高校优势学科建设工程资助项目(PAPD)
