摘要
目的:探讨经体外培养的出生后的猪牙源性间充质细胞成牙能力。方法:将体外培养第4代的猪牙胚细胞接种到胶原凝胶基质中,体外培养3 d后进行裸鼠体内移植,6 W后取材观察。结果:猪牙胚细胞原代培养过程中可见上皮样细胞与成纤维样细胞混杂存在,传代3次后上皮样细胞消失。细胞-胶原基质复合物取材结果显示细胞聚集的区域可见牙本质-牙髓复合体样结构。结论:经体外传代培养后未经体外诱导的牙胚细胞接种到胶原凝胶基质所构建的组织工程牙齿有牙本质-牙髓复合体样结构形成,说明牙胚细胞经体外培养后其所获得的发育信息仍可保持,是研究牙齿发育构建模型及组织工程牙齿的理想的种子细胞。
Objective： To study the odontogenesis ablility of pig dental mesenchymal cells cultured in vitro. Method： Seeding pig mesenchymal cells of passage 4 into collagen materials. Alter 3 days culture, the cell-collagen compounds were / implanted into nude mice to establish the odontogenesis ablility of the dental mesenchymal cells. 6 weeks later, implants were excised for histological analysis. Result： Epitbelium-like cells and fibroblast-like cells were concomitance in the primary culture, while epithelium-like cells disappeared after 3 passages culture in vitro. The results of the implants showed that dental-pulp complex structures formed in the place where cells had a high density. Conclusion：After a long time culture in vitro, dental mesenchymal cells can form dental-pulp complex structure after being seeded into collagen matrix. The resuits showed that after a long time culture in vitro, dental mesenchymal cells still have the odontogenesis ablility. Thus dental mesenchymal cells are ideal for tooth engineering.
出处
《临床口腔医学杂志》
2006年第5期259-261,共3页
Journal of Clinical Stomatology
基金
国家自然科学基金资助项目(30572046)
