摘要
目的:比较药物联合预处理和单种药物预处理对沙土鼠脑缺血再灌注损伤的脑保护作用。方法:沙土鼠43只,随机分为正常对照组(A)、缺血损伤组(B)、利多卡因及异丙酚联合预处理组(C)、异丙酚预处理组(D),利多卡因预处理组(E),C、D、E预处理组在脑缺血前24 h分别予利多卡因30 m g/kg及异丙酚100 m g/kg、异丙酚100 m g/kg、利多卡因30 m g/kg腹腔注射,对照组7只,余每组为9只,观察指标为SOD(超氧化物岐化酶)、G SH(谷光甘肽)的活性及M DA(丙二醛)、LDH(乳酸脱氢酶)、CPK(肌磷酸激酶)含量。每组随机取一左大脑皮层的1 mm×1 mm组织块作电镜,观察脑组织超微结构的改变。结果:各个药物预处理组的M DA、LDH、CPK的含量低于缺血损伤组(P<0.05,P<0.01或P<0.001),而SOD、G SH的活性高于缺血损伤组(P<0.05,P<0.01或P<0.001),而各个预处理组比较利多卡因及异丙酚联合预处理比单独预处理显示出来更好的保护作用(P<0.05或P<0.01)。与缺血再灌注组比较,各个药物预处理组在电镜超微结构均有改善,以利多卡因及异丙酚联合预处理组的改善较为明显。结论:缺血前24 h予药物预处理对沙土鼠的脑缺血再灌注损伤均有不同程度的减轻作用,并以联合预处理组的效果最为显著。
Objective：To compare the protective effect on cerebral ischemia reperfusion in gerbils between pretreatment with lidocaine combined with propofol and with either lidocaine or propofol. Methods; 43 Mongolian gerbils were randomly divided into five groups： （A）Control group, （B） ischemia group, （C） Lidocaine combined with Propofol pretreatment group, （D）Propofol pretreatment group, （E） Lidoeaine pretreatment group. Lidocaine 30 mg/kg and propofol 100 mg/kg were given intraperitpneally（ip） 24 h before isehemia in group C . Propofol 100 mg/kg or Lidocaine 30 mg/kg was given intraperitpneally（ip） 24 h before ischemia in D or E group ,respectively. There were nine gerbils in each group except seven in control group. LDH（lactate dehydrogenase） and CPK（Creatine phosphokinase）aetivities before decapitation were determined. The animals were then decapitated and forebrain cerebral cortex was removed to determine superoxid dismutase（SOD） and glutathione（GSH） activities and contents of malondial dehyde（MDA）. One left forbrain cerebral cortex （1 mm ×1 mm） was removed randomly in each group to observe the change of ultrastructure of cerebral tissue with electron microscope. Results;In every pretreatment group MDA,LDH and CPK were significantly lower than those in ischemia group（P〈0. 05,P〈0.01 or P〈0. 001）and activities of SOD and GSH were higher than those in ischemia group（P〈0.05 ,P〈0.01 or P〈0. 001）;There were significant difference （P〈0.05 or P〈0.01） between Lidocaine with propofol pretreatment group and the other prtreatment groups. Compared with ischemia-reperfusion group, the ultrastructures of cerebral tissue were improved to different degree in each pretreatment group. The improvement of the ultrastructure of cerebral tissue was better in Lidocaine with Propofol group than in the other two pretreatment groups. Conclusions：Pretreatment with either lidocaine or propofol 24h before ischemia showed protective effects on cerebral ischemia reperfusion injury to different degrees in gerbils, and the protective effect was most significant in Lidocaine combined with Propofol pretreatment group.
出处
《中国误诊学杂志》
CAS
2006年第9期1630-1633,共4页
Chinese Journal of Misdiagnostics
