摘要
目的观察不同时间点予利多卡因预处理对沙土鼠脑缺血再灌注损伤的保护作用及寻找利多卡因最佳预处理时间点。方法沙土鼠43只,随机分为正常对照(A)组、缺血损伤(B)组、利多卡因预处理(C^E)组,即脑缺血前48、24、12h予利多卡因30mg/kg腹腔注射。对照组7只,余每组为9只。观察指标为超氧化物歧化酶(SOD)、谷胱甘肽(GSH)的活性及丙二醛(MDA)、内皮素(ET)、降钙素基因相关肽(CGRP)的含量。每组随机取一左大脑皮层的1mm×1mm组织块作电镜,观察脑组织超微结构的改变。结果利多卡因预处理各组的SOD、GSH的活性高于缺血损伤组(P<0.01或P<0.05),而MDA、ET的含量低于缺血损伤组(P<0.01或P<0.05),CGRP的含量虽高于缺血组,但两者的差别不具有统计学意义(P>0.05)。利多卡因预处理各组的脑组织超微结构损伤改变不大或仅有轻微的改变,而缺血组脑组织超微结构表现出严重的损伤。结论缺血前48~12h予利多卡因预处理对沙土鼠的脑缺血再灌注损伤有不同程度的保护作用,这种作用与其增加体内抗氧化物质SOD、GSH的活性及拮抗ET的毒性有关。
Objective To observe the protection of Lidocaine pretreatment against cerebral ischemia-reperfusion injury in gerbils and search the optimized pretreatment time. Methods Forty-three Mongolian gerbils were randomly divided into five groups ： （A） Control group, sham operation. （ B ） ischemia group （ C-E ） Lidoeaine groups, Lidoeaine 30mg/kg was given intraperitpneally （ ip ） 48h （ C ） , 24h （D）, 12h （E） before ischemia. There were nine gerbils in each group except seven gerbils in control group. Animals was anesthetized with Urethane lg/kg ip. The animals in group B-E were exposed their bilatered common carotid arteries and clamped for ischemia for 10 min and then unclamped for reperfusion for 50min ;The animals in control group were exposed their bilatered common carotid arteries but not clamped. The animals were then decapitated and forebrain cerebral cortex was removed to determinate superoxid dismutase （SOD ） and glutathione （GSH） activities and contents of malondial dehyde （MDA）, endothelin （ET） and calcitonin gene-related peptide （ CGRP） .A left forebrain cerebral cortex （lmm x lmm） was removed in random in each group to observe the change of uhrastracture of cerebral tissue with electron microscope. Results In Lidocalne groups MDA and ET content was significantly lower than those in ischemia group （ P 〈 0.01 or P 〈 0.05） and SOD,GSH activities were higher than those in ischemla group （ P 〈 0.01 or P 〈 0.05） ;In Lidocaine group the CGRP content was higher than those in ischemia group but their differences were not significant in statics （ P 〉 0.05） .The ultrastructure of cerebral tissue was seriously damaged in ischemia-reperfusion group,and the ultrastructure of cerebral tissue were not damaged or only slightly damaged in Lidocaine pretreatment groups. Conclusion Lidocaine given 12 - 48h before cerebral ischemia has varying degree protection against cerebral ischemia-reperfusion injury.The protection has relation with the rise of SOD, GSH activities and the decrease of ET content, stabilize the membrane of cell.
出处
《四川医学》
CAS
2005年第9期927-929,共3页
Sichuan Medical Journal
基金
贵州省科委科学技术基金(No:20003011)
关键词
脑缺血再灌注损伤
利多卡因
预处理
脑保护
cerebral ischemia- reperfusion
Lidocaine
pretreatment
cerebral protection
