摘要
目的:探讨针刺作用的机理中是否存在着体液因素。方法:取新生大鼠心肌细胞进行原代培养。5天后应用荧光分子探针Fluo3AM染色。激光共焦扫描显微镜动态扫描观察,加入正常血清和电针刺“内关”“间使”的血清后,观察心肌细胞内Ca2+含量的变化。结果:加入正常血清后,细胞内的Ca2+含量会有一定程度的升高,经过一段时间后升高的Ca2+含量会趋向平稳;而再加入针刺血清后,可明显降低心肌细胞Ca2+的含量(P<0.01)。结论:针刺腧穴后的大鼠血清可使心肌细胞内Ca2+含量明显降低,为针刺效应的血清学机制提供了直接证据。
Objective To explore whether humoral factors play a role in the mechanisms of acupuncture. Methods Primary culture of myocardial cells of neonatal rats were carried out. Five days later, they were labeled by fluorescent molecular probe Fluo-3AM. Changes of Ca^2＋ contents in the cultured myocardial cells after addition of the normal rat serum or acupuncture-serum of the rat who received acupuncture at ＂Neiguan＂ （PC 6） and Jianshi （PC 5）, were dynamically observed by a confocal laser scanning microscopy. Results After addition of normal serum of the rat, the intracellular Ca^2＋ level increased to a certain degree, and then gradually tended to stability, which was significantly decreased by addition of the acupuncture-serum （P〈0.01）, Conclusion The serum of the rat who received acupuncture at aeupoints can decrease the Ca^2＋ level in cultured myocardial cells, which provides a direct evi dence for serum factors involving in acupuncture mechanism.
出处
《中国针灸》
CAS
CSCD
北大核心
2006年第5期367-370,共4页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金课题:90209024
