摘要
目的:探讨足阳明经穴针刺血清对家兔离体胃窦平滑肌细胞舒缩活动的影响,从离体细胞水平研究足阳明经与胃相关的特异性。方法:将45只家兔随机分为生理盐水组、阿托品模型组、针刺足阳明经穴组、针刺足少阳经穴组、针刺足太阳经穴组,每组9只。分组处理后,颈动脉采血制备针刺血清。另取正常家兔10只,取胃窦平滑肌,经Ⅱ型胶原酶消化后制成正常兔胃窦平滑肌细胞悬液。取1:2稀释的针刺血清与同体积的正常兔平滑肌细胞悬液作用,用戊二醛固定后在显微镜下以测微尺测定细胞长度。结果:阿托品模型组的血清能使正常兔胃窦平滑肌细胞舒张,细胞长度增加;针刺足阳明经穴组的血清可以促进正常兔胃窦平滑肌细胞收缩,细胞长度缩短,而针刺足少阳经穴组和针刺足太阳经穴组的血清对正常兔胃窦平滑肌细胞的长度影响不明显。结论:足阳明经穴针刺血清能使离体胃窦平滑肌细胞产生明显的收缩效应,从离体细胞水平进一步证实了足阳明经与胃相关的特异性。
Objective:To explore effects of serum after needling acupoints of Foot-Yangming Channel on diastolic and contractive activities of isolated gastric antrum smooth muscle cells in the rabbits,so as to study on specificity of correlation between Foot-Yangming Channel and stomach.Methods:45 rabbits were randomly divided into saline group,atropine model group,needling Foot- Yangming Channel's acupoint group,needling Foot-Shaoyang Channel's acupoint group and needling Foot-Taiyang Channel's acupoint group,9 rabbits each group.Blood was taken from the carotid for preparing acupuncture serum.And 10 normal rabbits were selected and their gastric antrum smooth muscle were taken and digested with type Ⅱ collagenase and then made as suspension of the normal rabbit's gastric antrum smooth muscle cells.Then 1:2 acupuncture serum was mixed with the same volume of the suspension of normal rabbit smooth muscle cells,and after fixation with glutaradehyde the cellular length was measured with micrometer under a microscope.Results:Serum of the atropine model group could extend normal rabbit's gastric antrum muscle cells with the length of cell increased;Serum in the needling Foot-Yangming Channel's acupoint group could promote contraction of normal rabbit's gastric antrum smooth muscle cells with the length of cell shortened,while serum in both needling Food-Shaoyang Channel's acupoint and needling Foot-aiyang Channel's acupoint groups did not significantly induce contractive effect on the cells.Conclusion:The rabbit serum after needling acupoints of Foot-Yangming Channel can induce significant contraction of isolated gastric antrum smooth muscle cells,which proves the specificity of correlation between Foot- Yangming Channel and stomach.
出处
《中医杂志》
CSCD
北大核心
2005年第4期264-266,共3页
Journal of Traditional Chinese Medicine
基金
国家自然科学基金(30171136)
湖南省教育厅重点项目(01A013)
