摘要
目的:探讨四逆汤预处理后诱导的心肌延迟预适应能否抑制心肌缺血再灌注(M I/R)后心肌细胞凋亡的发生及其可能机制。方法:SD大鼠分为正常对照组、假手术组、缺血再灌注组、四逆汤预处理组。缺血再灌注组采用经典大鼠冠脉结扎,缺血1 h,再灌1 h。四逆汤预处理组给予四逆汤灌胃(5 m l.kg-1.d-1)连续3天,末次灌药24 h后心肌缺血1 h,再灌1 h。流式细胞仪测定心肌细胞凋亡率,RT-PCR法检测bc l-xl mRNA/bc l-xs mRNA的表达情况并进行线粒体内凋亡诱导因子(AIF)的W estern b lotting分析。结果:四逆汤预处理24 h后可以减少M I/R后心肌细胞凋亡率,升高bc l-xlmRNA/bc l-xs mRNA表达的比值,减少AIF自线粒体的释放。结论:四逆汤诱导的延迟预适应可以减少M I/R造成的细胞凋亡,机制与其保护线粒体有关。
Objective： The present study was designed to determine the mechanism of myocardial cell apoptosis during delayed preconditioning induced by Sini decoction （SND）. Methods ： Sprage-Dawleytrats were divide into four groups ： control, sham, I/R and SND groups. Left anterior descending coronary artery （LAD）of the rats in I/R group were occluded for lh and reperfu^d for lh. SND group rates were pretreated with Sini decoction （5 g· kg^-1· d^-1 ） for three days and the last treatment was pretreated 24h before the index occlusion. Cell apoptosis was measured by flow cytometry, mRNA level of bcl-xl/bcl-xs was detected by RT-PCR and apoptosis in- ducing factor （AIF） was detected by western blotting. Results： SND pretreatment for 24 hours could decrease cell apoptosis, increase bcl-xl-bcl-xs mRNA ratio and decrease release of AIF from mitochondria. Conclusion： The delayed preconditioning induced by SND Can decrease myocardial cell apoptosis. The mechanism is related to mitochondria protection.
出处
《中药材》
CAS
CSCD
北大核心
2006年第4期345-348,共4页
Journal of Chinese Medicinal Materials
基金
广东省中医药管理局基金(1040066)
广东药学院科研基金(启动)项目
