摘要
目的 考察四逆汤有效部位抗心肌缺血-再灌注损伤的作用。方法 实验动物随机分为正常组、模型组、四逆汤组、四逆汤有效部位组。大鼠心脏进行Langendorff灌流，通过控制灌流液流量建立心肌缺血-再灌注损伤模型，用药组在灌流液中加入相应药液，测定再灌注后50rain的冠脉流量、心肌收缩力和再灌注1min内心律失常发生率。小鼠ig给药3d后ip垂体后叶素（20U／kg）造模，记录0～8min的心电图，并取心肌测SOD活性、MDA及LA水平。结果 四逆汤和四逆汤有效部位均可不同程度地提高大鼠心肌缺血再灌注损伤心肌收缩力恢复率、降低再灌注1min心律失常发生率，四逆汤有效部位对心肌收缩力的恢复作用较好，四逆汤对心律失常发生的抑制较好；四逆汤和四逆汤有效部位均可明显地降低小鼠心肌缺血-再灌注损伤过程中的心电图J点抬高，明显提高心肌组织SOD活性，降低MDA和LA水平，四逆汤和四逆汤有效部位作用无明显差异。结论 四逆汤有效部位可以显著地改善心肌缺血-再灌注损伤过程中的氧化损伤和抑制无氧酵解，改善心肌收缩功能和心律失常。
Objective To study the anti-myocardial ischemia-reperfusion injury effect of Sini Decoction＇s active fraction （SNDAF）. Methods Experimental animals were randomly individed into nor mal, model, Sini Decoction （SND）, and SNDAF groups. Rat hearts were perfused by the Langendorff method. The model of myocardial ischemia reperfusion injury was reproduced by adjusting the flow of per fused liquid. The perfused liquid of normal and model groups was KH buffer saturated by oxygen. The perfused liquid with SND or SNDAF was mixed with KH buffer. The coronary flow and contract power of myocardium at 0 rain of ischemia and 1, 5, 10, 20, 30, 40, and 50 min of reperfusion were tested, respec tively. The incidence of reperfusion arrhythmias （IRA） in the first 1 minute of reperfusion was calculated. Mice were given drugs by ig for 3 d and then myocardial ischemia-reperfusion models were established by ip pituitrin （20 U/kg）. ECG of mice was recorded at 0-80 min after ip administration. SOD activity, and the contents of MDA and LA in myocardium of mice were measured. Results Both SND and SNDAF could reinforce myocardial contract and reduce the IRA in the first minute of reperfusion. SND had better effect on IRA than that of SNDAF. SNDAF had better effect on myocardial contract than that of SND. SND and SNDAF could significantly drop ECG J point raise induced by ischemia, and increase SOD activity, and decrease contents of MDA and LA significantly in ischemia myocardium. There were no significant difference between SND and SNDAF. Conclusion SNDAF could improve oxidative injury and suppress ischemia of myocardium, reinforce myocardial contract, and reduce the incidence of arrhythmias during myocardia ischemia-reper fusion.
出处
《中草药》
CAS
CSCD
北大核心
2006年第1期77-81,共5页
Chinese Traditional and Herbal Drugs
基金
"211工程"重点建设项目课题(98176)
广东省高教厅资助
广东省中医药管理局科研基金资助项目(100118)
