摘要
目的了解三氧化二砷对激素非依赖性前列腺癌PC-3细胞株细胞增殖、细胞凋亡及bcl-2、bax基因表达的影响。方法分别以1、2、3、6、10μmol/L的三氧化二砷作用于PC-3细胞,48h后对细胞增殖活性、细胞凋亡及细胞bcl-2、bax基因表达的变化进行检测。结果各浓度的三氧化二砷均可抑制PC-3细胞增殖,并具有剂量依赖性。3、6、10μmol/L的三氧化二砷还可诱导PC-3细胞凋亡,计算细胞凋亡率分别为11．8%、12．7%、29．6%。逆转录聚合酶链反应(RT-PCR)检测PC-3细胞中bcl-2、bax表达的相对强度,发现bcl-2的表达强度随三氧化二砷浓度的升高而逐渐降低(P＜0．01),而bax表达强度变化不明显(P＞0．05)。结论三氧化二砷可有效抑制PC-3细胞增殖,诱导PC-3细胞凋亡,这一过程与三氧化二砷抑制PC- 3细胞中bcl-2的表达有关。
Objective To investigate the effect of arsenic trioxide on cell proliferation, cell apoptosis, bcl-2, bax gene expression in hormone-refractory prostate cancer PC-3 cells. Methods After PC-3cells were incubated with different concentration of arsenic trioxide for 48 hours. The cell variability, cell apoptosis and bcl-2, bax expression in PC-3 cells were dectected. Results Arsenic trioxide could inhibit cell proliferation and induce apoptosis in PC-3 cells. When the concentration of arsenic trioxide were 3、 6、 10μmol/L,the apoptotic rate in PC-3 cells were 11.8%、 12.7%、 29.6% respectively, and the bcl-2 gene expression was reduced as the concentration of arsenic trioxide increased, Conclusion Arsenic trioxide can effectively inhibit cell proliferation and induce apoptosis in hormone-refactory prostate cancer PC-3 cells, this may result from its action of reduce bcl-2 gene expression in PC-3cells.
出处
《中国男科学杂志》
CAS
CSCD
2006年第4期44-47,共4页
Chinese Journal of Andrology
