摘要
目的:观察三氧化二砷对人食管癌细胞株Eca-109细胞裸鼠体内移植瘤生长的影响。方法:实验于2005-07/2006-07在解放军第四五八医院肿瘤中心实验室完成。①实验材料及方法:将体外培养的人食管癌细胞系Eca-109接种于裸鼠皮下,建立移植瘤模型。将20只裸鼠按随机数字表法分为4组(n=5),即生理盐水对照组、1μmol/L三氧化二砷组、4μmol/L三氧化二砷组和8μmol/L三氧化二砷组。各组均于瘤块移植后48h取不同剂量的药物0.2mL腹腔注射,给药5周。②实验评估:检测各组实体肿瘤生长情况并行常规病理观察。结果:20只Eca-109细胞移植裸鼠全部进入结果分析,无脱失。①裸鼠移植瘤生长情况:8μmol/L三氧化二砷组裸鼠对Eca-109细胞移植瘤的抑制率明显高于1μmol/L,4μmol/L三氧化二砷组和生理盐水对照组(P<0.05),其他各用药组之间移植瘤体积差异无显著性意义(P>0.05)。移植后4周和5周时,8μmol/L三氧化二砷组裸鼠Eca-109细胞移植瘤的抑制率分别为68.5%和80.7%,抑制作用呈剂量依赖性。②移植瘤组织病理学变化:生理盐水对照组细胞呈多角形,核圆形,核浆比例大,核分裂多见,可见异常核分裂。用药组瘤细胞孤立,核分裂少见,可见核染色质固缩,核边聚,核碎裂等凋亡现象。结论:三氧化二砷对人食管癌裸鼠移植瘤具有明显的抑制作用,并且呈一定的剂量依赖性。
AIM： To explore the antitumor effect of arsenic trioxide （As2O3） on human carcinoma of esophagus cell strain Eca-109 transplanted in nude mice. METHODS： The experiment was carded out in the Central Laboratory of Oncology at the 458 Hospital of Chinese PLA from July 2005 to July 2006. ①The human carcinoma of esophagus cell line Eca-109 were cultured in vitro and transplanted to the subcutaneous tissues of nude mice for tumor models. Twenty nude mice were divided into four groups at random （n =5）, then the mice with tumors were respectively treated with saline and different concentrations （1; 4, 8μol/L） of As203 for 5 weeks, 0.2 mL by intraperitoneal injection. ②The growth of solid tumor was detected and routine pathological examination was conducted. RESULTS： Twenty nude mice transplanted with Eca-109 cells were all involved in the result analysis.①The inhibition rate of tumor growth in the group of 8 μmol/L As203 was significantly higher than other treated groups and normal saline group （P 〈 0.05）. There was no significant difference between other treated groups （P 〉 0.05）. At the 4^th and 5^th weeks of transplantation, the inhibition rate of tumor growth in the group of 8 μmol/L As2O3 was 68.5% and 80.7%, respectively, which indicated the dose-dependent manner. ②The pathological examination showed that, tumor cells in normal saline group were polygonal and abnormal karyokinesis was common, with round cellular nucleus and high karyoplasmic ratio. In treated groups, tumor cells were isolated and karyokinesis was few, cell apoptosis was also found with the appearances of nuclear chromatinic pyknosis, nuclear divergence and fragmentation. CONCLUSION- As203 can inhibit the carcinoma of esophagus in nude mice obviously and this effect presents a dose-dependent manner.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第34期6762-6764,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
中国博士后科学基金(20060400771)~~
