期刊文献+

与黄瓜抗枯萎病基因连锁的RAPD标记 被引量:16

Identification RAPD Markers Linked to Fusarium Wilt Resistance Gene in Cucumber
在线阅读 下载PDF
导出
摘要 以黄瓜抗枯萎病亲本WIS2757和感枯萎病亲本津研2号及其F2分离群体为试材,采用分离群体分组分析法(BSA)进行了与黄瓜抗枯萎病基因连锁的分子标记研究。运用RAPD技术,利用780条RAPD引物对抗、感亲本进行筛选,其中有113条引物在两亲本之间表现多态性,但仅有引物S49在两组间多态性标记与亲本的多态性标记相同。经F2单株分析,引物S49扩增出的特异DNA片段与WIS2757抗黄瓜枯萎病基因连锁,遗传距离为14 cM。DNA标记条带大约为300 bp,定名为S49-300。 Using RAPD primers with BSA technique screened the RAPD markers linked to Fusarium Wilt resistance gene in Cucumber. The results showed that 113 arbitrary primers can amplified different polymorphism bands between two parents, primer S49 can amplify a specific polymorphism band which is linked to Fusarium Wilt resistance in Cucumber, the genetic distance is 14 cM, and the band is named S49-300.
出处 《华北农学报》 CSCD 北大核心 2006年第2期121-123,共3页 Acta Agriculturae Boreali-Sinica
基金 北京市自然科学基金(5052001 5062008)国家自然科学基金资助(30471186)
关键词 黄瓜 枯萎病 抗性基因 RAPD Cucumber Fusarium Wilt Resistance gene RAPD
  • 相关文献

参考文献14

  • 1李传宝.黄瓜枯萎病药剂防治试验[J].广西农业科学,2003,34(4):40-41. 被引量:5
  • 2朱振东,周荣华,贾继增.小麦品系抗小麦白粉病基因分子标记鉴定[J].作物学报,2005,31(8):977-982. 被引量:12
  • 3Gupta P K,Varshney R K,Sharma P C,et al.Molecular markers and their applications in wheat breeding[J].Plant Breeding,1999,118:369-390.
  • 4Young N D.A cautionusly optimistic vision for marker-assisted breeding[J].Molecular Breeding,1999,5:505-510.
  • 5Thomas Horejsi,Jack E,Staub Claude Thomas.Linkage of random amplified polymorphic DNA markers to downy mildew resistance in cucumber (Cucumis sativus L.)[J].Euphytica,2000,115:105-113.
  • 6张桂华,杜胜利,王鸣,马德华.与黄瓜抗白粉病相关基因连锁的AFLP标记的获得[J].园艺学报,2004,31(2):189-192. 被引量:57
  • 7Martyn R D.An aggressive race of Fusariumoxysporum Fsp niveum new to the United States[J].Plant Disease,1985,69:1007.
  • 8Sasaki A.Green revolution:amutantgibberellinsynthesis gene in rice new insight into the rice variant that helped to avert famine over thirty years ago[J].Nature,2002,416:701 -702.
  • 9Van Ooijen J W,Voorrips R E.JoinMap3.0,Software for the calculation of genetic linkage maps.Plant Reseach International,Wageningen,the Ntherlands.2001.
  • 10Paran I,Kesselli R,Michelmore R W.Identification of restriction fragment length polymorphism and random amplified polymorphic DNA markers linked to downy mildew resistance genesin lettuce,using near isogenic lines[J].Genome,1991,34:1021-1027.

二级参考文献28

  • 1孔令让,董玉琛.粗山羊草抗小麦白粉病基因遗传多样性的研究[J].作物学报,1997,23(2):176-180. 被引量:26
  • 2马德伟.厚皮甜瓜东移研究[J].中国蔬菜,1985,(4):10-13.
  • 3William J G K, Kubelik A R, Livak L J,et al. DNA polymorphism arbitrary primers are useful as genetic marker[J]. Nucleic Acids Research, 1990,18(22):6531 - 6535.
  • 4Martin G B. Rapid identification marker linked to Pseudomonas resistance gene in tomato by using random primer and near-isogenic line [J]. Proc Natl Acad Sci USA, 1991,88:2336 - 1436.
  • 5Young N D. Use of isogenic linesand simultaneous probing to identify DNA markers tightly linked to the Tm-2a gene in tomato[J] . Genetics, 1988,120:579 - 585.
  • 6Michelmore R W, Paran I, Keseli R V. Identification of markers linked to disease resistance gene by bulked segregant analysis:a rapid method to detect markers in specific genomic regions by using segregating population [J].Proc Natl Acad Sci USA , 1991,88:9828 - 9842.
  • 7Patrick W. Identification of a randomly amplified polymorphic DNA marker linked to the Fom 2 Fusarium wilt resistance gene in muskmelon MR-1[J]. Molecular Plant Pathology, 1995,85(10) :1245 - 1249.
  • 8Zheng X Y, David W W. Randomly amplified polymorphic DNA markers linked to Fusarium wilt resistance in diverse melons [J]. Hortscience, 2000, 35 (4):716-721.
  • 9Epinat C M, Pitrat, Bertrand F. Inheritance of resistance of five melon lines to Powdery mildews [J]. Euphytic, 1993, 65:135-144.
  • 10Floris E,Alvarez J M. Genetic analysis of resistance of three melon lines to Sphaerotheca fuliginea [J]. 1995,67:181 - 186.

共引文献101

同被引文献450

引证文献16

二级引证文献111

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部