摘要
目的建立高效液相色谱法测定通窍鼻炎片中5-O-甲基维斯阿米醇苷的含量。方法Kromassil C18色谱柱,5μm,250×4.6mm,甲醇-水-四氢呋喃(38∶62∶1)为流动相,流速为1.0ml.min-1,检测波长254nm。结果本法线性关系良好(r=0.9998)。平均加样回收率100.07%,RSD=1.39%(n=5)。结论本法简便易行,结果准确,重现性好。可用于通窍鼻炎片的质量控制。
OBJECTIVE To establish a HPLC method for determination of 5-O-MethylviSammioside in Tongqiao Biyan Tablets. METHODS The Kromassil C18 column（5μm,250×4. 6mm） was used,the mobile phase was methanol-water-tetrahydrofuran（38：62：1）. Flow rate was 1.0ml ·min^-1. Detection wavelength was at 254nm. RESULTS The method had a good linear dependence （r=0. 9998）. The average recovery was 100. 07% （ RSD=1.39%, n=5）. CONCLUSION The method is simple,rapid,accurate and reliable,and can be used to control quality of Tongqiao Biyan Tablets.
出处
《齐鲁药事》
2006年第4期222-223,共2页
qilu pharmaceutical affairs
