摘要
目的建立用高效液相色谱法测定复方三七注射液中三七总皂苷含量的方法。方法对样品的提取条件和色谱条件进行方法学研究。采用Waters Symetry C18色谱柱,以乙腈—水二元梯度为流动相,检测波长为203nm。结果三七皂苷R1进样量在1.5μg^15μg范围时,相关系数r=0.9998;人参皂苷Rg1进样量在2.8μg^28μg范围时,相关系数r=0.9999;人参皂苷Rb1进样量在4.1μg^41μg范围时,相关系数r=0.9999。人参皂苷Rg1、Rb1和三七皂苷R1的回收率分别为99.3%、95.7%和97.5%,RSD分别为1.2%、1.3%、2.0%。结论本法简便、快速、准确,重现性好,可作为复方三七注射液的质量控制。
OBJECTIVE To establish a method for the determination of the contents of the Panax notogineseng in Compound Sanqi Injection（CSI） by HPLC. METHODS To select the method of extraction for the sample and chromatographic conditions. The Waters Symetry C18 column was used with the mobile phase of acetonitrile-water, the wavelength of the detection was set at 203nm. RESULTS A good linearity for notoginsenoside R1 was in the range of 1.5-15μg（ r =0. 9998）, 2.8 28μg（ r=0. 9999） for ginsenoside Rg1 and 4.1-41μg （r=0. 9999） for ginsenoside Rb1, The average recoveries were 99. 3%, 95.7 % and 97.5 %, the RSD were 1.2%, 1.3 % and 2. 0%,respectively. CONCLUSION the method is simple, rapid, accurate and with good reproducibility and can be used for the quality control of CSI.
出处
《齐鲁药事》
2006年第4期216-218,共3页
qilu pharmaceutical affairs
