摘要
目的提取带形蜈蚣藻硫酸多糖,研究其理化性质和抗病毒活性。方法用热水浸提、乙醇沉淀法提取带形蜈蚣藻硫酸多糖、再经DEAE C-52纤维素离子交换柱层析得到2个较纯组分。并对其进行了单糖组成和硫酸基含量等理化性质分析。采用细胞病变(cytopathic effect,CPE)抑制法和四甲基偶氮唑盐(MTT)比色法,研究带形蜈蚣藻多糖的抗病毒活性。结果带形蜈蚣藻多糖主要由半乳糖组成,还含有少量的葡萄糖、木糖等,其硫酸基含量达到15%以上;该多糖具有较好的抗病毒活性,其中经提纯的两个组分抗HSV-1对细胞病变的半抑制浓度(IC50)分别为7.81,3.91μg.mL-1,选择(指数)SI值分别大于128,256。结论带形蜈蚣藻硫酸多糖抗CoxB3和HSV-1病毒活性均比较好。
Objective To extract the sulfated polysaccharides from Grateloupia turuturu Yamada and to studyits physico-chemical properties and antiviral activities. Methods The sulfated polysaccharides were extracted from Grateloupia turuturu Yamada by hot water extraction and alcohol precipitation. Two pure polysaccharides were obtained by DEAE-52 cellulose column chromatography. The physico-chemical properties including the composition of the glycoconjugate and the content of the sulfate were analyzed. The antiviral effects of the samples on HSV-1, Cox3 and their cytotoxicity to Veto, Hela cell were studied by cytopathic effect（CPE） reduction assay and MTT colorimetric assay. Results The results showed that the glycoconjugate was composed of Gal and slight Glc, Xyl etc. The content of the sulfate group was more than 15%. And the polysaccharides showed good antiviral activities, especially the pure polysaccharides. Their 50% inhibited concentrations （IC50） on HSV-1 were 7.81 and 3.91μg · mL^-1, selectivity index（SI）values were larger than 128 and 256, respectively. Conclusion The sulfated polysaccharides from Grateloupia turuturu Yamada have good antiviral effects on HSV-1 and Cox3.
出处
《中国海洋药物》
CAS
CSCD
2006年第2期12-16,共5页
Chinese Journal of Marine Drugs
基金
广东省"十五"重大科技专项(2002A3050503)
广东省自然科学基金研究团队项目粤科基办【2003】11-039213
关键词
带形蜈蚣藻
硫酸多糖
抗病毒活性
Grateloupia turuturu Yamada
sulfated polysaccharides
antiviral activities
