摘要
目的:探讨骨肉瘤中survivin基因在转录水平及蛋白水平的表达特点及与肿瘤细胞增殖凋亡的关系。方法:骨肉瘤组采用2003年1月至2004年12月骨肉瘤标本48例,对照组为骨软骨瘤标本17例。采用逆转录PCR法、免疫组织化学染色技术、DNA原位末端标记法(TUNEL)检测组织中survivin mRNA及蛋白表达、PCNA表达及细胞凋亡情况。结果:骨软骨瘤组survivn mRNA表达率为17.6%(3/17),蛋白表达率为11.8%(2/17),骨肉瘤组survivin mRNA表达率为93.8%(45/48),蛋白表达率为72.9%(35/48),均显著高于骨软骨瘤组。骨软骨瘤组PCNA阳性表达率为41.2%(7/17),骨肉瘤组为77.1%(37/48),显著高于骨软骨瘤组(P<0.05)。骨软骨瘤组TUNEL染色阳性率为82.4%(14/17),骨肉瘤组为56.3%(27/48),统计分析有显著区别。survivin蛋白阳性表达与患者性别、骨肉瘤病理分型及临床分期无关,与肿瘤病理分级及远处转移成显著相关。结论:survivin特异性表达于骨肉瘤,与肿瘤病理分级及远处转移显著相关,survivin可能是诊断及评价骨肉瘤预后的重要参考指标。
Objective. To investigate the expression of survivin on transcription and protein level in osteossrcoma and the relationship between survivin expression with cell apoptosis and proliferation. Methods： Forty - eight patients suffered from osteosarcoma were included in this experiment and the control group included 17 cases suffered from osteochondroma. The expressions of survivin mRNA, survivin protein, PCNA and cell apoptosis were detected by RT - PCR, histochemistry staining and TUNEL staining. Results： The expression rate of sruvivin mRNA and protein were 17.6%（3/17） and 11.8%（2/17）, respectively, in osteochondroma and 93. 8%（45/ 48） and 72. 9%（35/48）, respectively, in osteosarcoma, there were remarkably difference between two groups. The expression rate of PCNA in osteosarcom（77. 1%, 37/48） was higher than that in osteochondroma（41. 2%, 7/17）. The positive rate of TUNEL in osteosareom（56. 3%, 27/48） was lower than that in osteochondroma （82. 4%, 14/17）. The protein expression of survivin in osteosarcoma was not correlated with patient＇s sex, pathological type and clinical stage, but was correlated with pathological grade and metastasis. Conclusion： Survivin was expression in osteosarcoma specifically. The expression of survivin is correlated with pathological grade and metastasis. Survivin may be a marker for the diagnosis and evaluation of osteosareorna.
出处
《西南国防医药》
CAS
2006年第2期121-123,共3页
Medical Journal of National Defending Forces in Southwest China
