摘要
目的研究三元一体包装ClO2消毒粉剂杀菌效果和毒性。方法采用悬液定量杀菌试验、能量试验和动物毒性试验进行了观察。结果以含25 mg/LClO2消毒液对悬液内大肠杆菌作用5 min,平均杀灭率为99.96%;12.5 mg/LClO2消毒液对金黄色葡萄球菌作用5 min,平均杀灭率为99.92%;50 mg/LClO2消毒液对枯草杆菌黑色变种芽孢作用10min,平均杀灭率为99.93%;含25%以上小牛血清对其杀灭大肠杆菌效果有轻度影响。以200mg/LClO2消毒液作用5 min,完全破坏悬液内HBsAg的抗原性。将三元一体包装ClO2消毒粉剂于54℃存放120 d,ClO2的下降率为8.2%。小鼠急性经口LD50值大于5000 mg/kg,以100 mg/LClO2消毒液对家兔皮肤刺激试验指数为0.63,属于轻度刺激性。结论三元一体包装ClO2消毒粉剂性能稳定,对细菌繁殖体和细菌芽孢具有良好的杀菌效果,属无毒、低刺激性消毒剂。
Objective： To study the germicidal efficacy and toxicity of three components Chlorine Dioxide disinfection powder, Methods： Suspension quantitative germicidal test, capacity test and animal toxicity test were used to carry out observation on them. Results： The average killing rates of Eseheriehia eoli in suspension exposed to the disinfectant solution containing Chlorine Dioxide 25mg/L for 5 min, and Staphylococcus aureus exposed, ed to the solution containing Chlorine Dioxide 12.5mg/L for 5 min were 99.96% and 99.92% respectively. The average killing rate of spores of Bacillus subtilis var. niger exposed to the solution containing Chlorine Dioxide 50mg/L for 10 min was 99.93 %. 200 mg/L Chlorine Dioxide solution with a 5 min contact time could destroy completely the antigenicity of HBaAg in suspension. After the three components Chlorine Dioxide powder was stored at 54 ℃; for 120 days, its content decreased by 8.2 %. The mouse acute oral LD50 was above 5000mg/kg, the rabbit skin irritation test index of .solution containing Chlorine Dioxide 100mg/L was 0.63, which belonged to mild irritation. Conclusions： Three components Chlorine Dioxide disinfected powder are chemically stable and kill effectively the vegetative forms of bacteria and bacterial spores. This disinfectant belongs to practically non- toxic class and is slightly irritative to skin.
出处
《泰山医学院学报》
CAS
2005年第4期339-342,共4页
Journal of Taishan Medical College
关键词
ClO2消毒粉剂
大肠杆菌
金黄色葡萄球菌
枯草杆菌黑色变种芽孢
HBsAg抗原性
three components Chlorine Dioxide disinfection powder
escheriehia coli
staphylococcus aureus
spores of Bacillus subtilis var. niger
antigenicity of HBsAg
