摘要
目的用高通量的组织芯片技术,对胰腺癌组织、癌旁组织及正常组织的p53、p16和环氧化酶-2(COX-2)蛋白异常表达进行分析,探讨其相关性及临床意义。方法利用组织芯片技术结合免疫组化法检测104例胰腺癌组织、癌旁组织和正常组织中p53、p16和COX-2蛋白的表达。结果胰腺癌组织中p53、p16、COX-2的阳性表达率均显著高于非癌组织(均P<0.05)。p53与p16蛋白表达不存在相关性(P>0.05),p53与COX-2、p16与COX-2蛋白表达均存在相关性(均P<0·05)。p53和COX-2的交互作用对于胰腺癌的发病率影响具有统计学意义(OR=19.686)。结论组织芯片技术是大规模平行检测多基因蛋白表达的一种有效方法;胰腺癌的发生与p53、p16和COX-2等有关,尤其后者的交互作用可能起了更重要的作用。
Objective To detect the expression of tumor suppressor protein p53, cyclin-dependent kinase inhibitor p16, and cyclooxygenase 2 （COX-2） in pancreatic cancer by tissue microarray and investigate the correlation among these three genes. Methods 104 specimens of dssues, including pancreatic cancer tissue, non-cancer tissues not more than 1.5 cm from the cancer, and normal tissues, underwent microarray examination and immunohistochemistry to detect the expression of p.53, p16, and COX-2. The correlation among these 3 genes was analyzed. Results p.53, p16, and COX-2 were all significantly highly expressed in the cancerous tissues in comparison with other tissues. P53 and p16 were both significantly correlated with COX-2 （ both P 〈 0.05 ）, however, there was not a correlation between p.53 and p16 （P 〉 0.05 ）. There was a reciprocal relationship between p53 and COX-2 （ P 〈 0.05, OR = 19. 686） influencing the pathogenesis of pancreatic cancer. Conclusion Tissue microarray technique is effective method to detect multiple gene protein expression. Pathogenesis of pancreatic cancer is associated with p53, p16, and COX-2.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2006年第14期939-942,共4页
National Medical Journal of China
基金
国家"863"计划功能基因组与生物芯片重大专项基金资助(2002AA2Z2021)
关键词
胰腺肿瘤
蛋白质P53
蛋白质P16
环氧化酶-2
组织芯片
Pancreatic neoplasms
Tumor suppressor protein p53
Cyclin-dependent kinase inhibitor p16
Cyclogenase 2
Tissue microarray
