摘要
甘草酸（glycyrrhizic acid，GL）及其衍生物是一类具有重要生理活性的化合物。利用酶转化GL可以生成具有更高生理活性的单葡萄糖醛酸甘草次酸（glycyrrhetyl 3-O-mono-β-D—glucuronide，GAMG）。为了深入研究该酶对GL的生物转化特性，该文建立了一种能同时检测GL及其酶转化产物GAMG的高效液相色谱分析方法。采用Apollo C18分析柱，流动相V（甲醇）：V（水）：V（冰醋酸）=75：25：5，流速1．0mL／min，紫外检测波长254nm。在进样量为0．2—20μg内成良好的线性关系，GL的加样回收率为94．3％-103．2％，相对标准偏差为0．85％-2．53％；GAMG的加样回收率为92．6％-98．7％，相对标准偏差为1．45％-3．25％。方法准确、简捷、耗材低廉。
Glycyrrhizic acid（GL） and its derivatives have various important physiological activities. GL can be transformed by enzyme to glycyrrhetyl 3-O-mono-β-D-glucuronide （GAMG） which is more physiologically active than GL. In order to characterise the enzymatic reaction, a method of reversed phase high performance liquid chromatography （RP-HPLC） was developed for the simultaneous determination of GL and GAMG in the enzymatic transformation system. The analysis could be performed with Apollo C18 column using a mobile phase of V（ methanol ） ： V（ water ） ：V（acetic acid ） = 75：25：5 at a flow rate of 1.0 mL/min with UV detection at 254 nm. The linear ranges of GL and GAMG were both between 0.2 -20 μg. The recoveries of GL and GAMG were 94. 3% - 103.2% and 92.6% -98.7% with relative standard deviations of 0.85% -2.53% and 1.45% -3.25% （n =3） respectively. This method is accurate, simple, rapid and cheap for simultaneous determination of GL and GAMG in the enzymatic transformation system.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2006年第4期355-357,共3页
Fine Chemicals
基金
高等学校博士学科点专项科研基金(20040291004)
国家重大基础研究项目(2003CB716000)~~
关键词
高效液相色谱法
甘草酸
单葡萄糖醛酸甘草次酸
酶转化
high performance liquid chromatography
glycyrrhizic acid
glycyrrhetyl 3-O-mono-β-D- glucuronide
enzymatic transformation
