摘要
建立了同时测定银翘解每片中氯原酸、甘草酸和甘草次酸的高效毛细管电泳法，电解缓冲液为20mmol／L磷酸二氢钠和5mmol／L硼砂的混合溶液（pH7.0）。方法简便快速，具有良好的精密度、回收率和线性关系，并测定了很翘解毒片中3组分的含量。
A simple, rapid and reproducible capillary electrophoretic method was developed for the determination of chlorogenic acid, glycyrrhizin and glycyrrhetinic acid in Yinqiaojiedupian. The buffer solution used in this method was a mixture of 20 mmol/L NaH2PO4 and 5 mmol/L Na2B4O7 (pH 7. 0). The other conditions were as follows: applied voltage, 20 kV (positive to negative polarity) ; L= 60 cm; Leff= 55cm; i. d. = 75μm; λ= 254 nm ; temperature, 25℃ ; injection time, 5 s hydrostatic. The linear calibration range was 1. 568-100. 4 mg/L for glycyrrhetinic acid, 8. 000-128. 0 mg/L for glycyrrhizin and 8. 080-129. 3 mg/L for chlorogenic acid, and recoveries were 98. 0%-103% for glycyrrhetinic acid, 97. 3%-102% for glycyrrhizin and 97. 3%-100% for chlorogenic acid. The relative standard deviations (n = 3) were 0.2%-%1. 3 % (intra-day) and 1. 9 %-3. 0% (inter-day) for glycyrrhetinic acid, 0. 5 %-1. 2 % (intra-day) and 1.9%-%2. 1 % (inter-day) for glycyrrhizin and 0. 7 %-1. 3 % (intra-day) and 1. 6%-2. 1 % (inter-day) for chlorogenic acid. The contents of these three acids in Yinqiaojiedupian were successfully determined within 10 min.
出处
《色谱》
CAS
CSCD
北大核心
1999年第2期193-195,共3页
Chinese Journal of Chromatography
基金
国家自然科学基金!39770904
关键词
银翘解毒片
氯原酸
甘草酸
甘草次酸
测定
HPLC
high performance capillary electrophoresis, Yinqiaojiedupian, chlorogenic acid, glycyrrhizin,glycyrrhetinic acid
